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활성 산소가 Adenosine Triphosphate에 의한 조골 세포내 Ca2+ 활성도 변화에 미치는 영향

Other Titles
 Effects of Reactive Oxygen Species on ATP-Induced Intracellular Ca2+ Activity in Osteoblasts 
Authors
 김진호  ;  권태동  ;  남순호  ;  서창국  ;  홍용우 
Citation
 Journal of Korean Society of Anesthesiologist (대한마취과학회지), Vol.44(5) : 544-554, 2003 
Journal Title
 Journal of Korean Society of Anesthesiologist (대한마취과학회지) 
ISSN
 0302-5780 
Issue Date
2003
MeSH
ATP ; Ca2+ activity ; H2O2 ; IP3 receptor ; osteoblast
Keywords
ATP ; Ca2+ activity ; H2O2 ; IP3 receptor ; osteoblast
Abstract
Background: The physiological activity of osteoblsts is known to be closely related to increased intracellular Ca2+ activity ([Ca2+]i) in osteoblasts. The cellular regulation of ([Ca2+]i) in osteoblasts is mediated by Ca2+ movements associated with Ca2+ release from intracellular Ca2+ stores, and transmembrane Ca2+ influx via Na+-Ca2+ exchanger, and Ca2+ ATPase. Reactive oxygen species, such as H2O2, play an important role in the regulation of cellular functions, and act as signaling molecules or as toxins in cells. Methods: Osteoblasts were isolated from the femurs and tibias of neonatal Sprague-Dawley rats, and cultured for 7 days. The cultured osteoblasts were loaded with a Ca2+-sensitive fluorescent dye, Fura-2 AM ester, and fluorescence images were monitored using a cooled CCD camera. Ca-spike changes upon ATP application were checked for (1) osteoblasts in Ca2+-free and 2.5 mM CaCl2 normal Tyrode solution, (2) osteoblasts in which the Ca2+ of the endoplastic reticulumin had been depleted with ryanodine, thapsigargin ord caffein, and (3) osteoblasts pretreated with H2O2, in which the expression of IP3 receptor was checked by Western blotting. Results: ATP increased intracellular free Ca2+ regardless of extracellular Ca2+ concentration. When the intracellular Ca2+ store was depleted, the level of increased Ca2+ activity by ATP was suppressed. H2O2 sustained the Ca2+ increase induced by ATP. The expression of IP3 receptor was enhanced by H2O2. Conclusions: H2O2 modulates intracellular Ca2+ activity in osteoblasts by increasing Ca2+ release from the intracellular Ca2+ stores.
Files in This Item:
T200302650.pdf Download
DOI
OAK-2003-00166
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Anesthesiology and Pain Medicine (마취통증의학교실) > 1. Journal Papers
Yonsei Authors
Kweon, Tae Dong(권태동) ORCID logo https://orcid.org/0000-0002-5451-1856
Kim, Jinho(김진호)
Nam, Soon Ho(남순호)
Hong, Yong Woo(홍용우)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/113326
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