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Calcium-Dependent Apoptotic Gene Expression in Cerulein-Treated AR42J Cells

Authors
 JI HOON YU  ;  HYEYOUNG KIM  ;  KYUNG HWAN KIM 
Citation
 ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, Vol.1010 : 66-69, 2003 
Journal Title
 ANNALS OF THE NEW YORK ACADEMY OF SCIENCES 
ISSN
 0077-8923 
Issue Date
2003
MeSH
Acute Disease ; Apoptosis/drug effects ; Apoptosis/genetics ; Apoptosis/physiology* ; Calcium/physiology* ; Calcium Signaling/drug effects ; Calcium Signaling/physiology* ; Cell Line ; Ceruletide/pharmacology* ; Gene Expression Regulation ; Gene Expression Regulation, Neoplastic/drug effects* ; Humans ; Pancreatitis
Keywords
15033695
Abstract
Elevated Ca2+ concentrations within the pancreatic acinar cells represent a risk factor for the development of acute pancreatitis. Apoptosis is an important characteristic of pancreatitis, with induction of apoptotic genes and intraceullar increase of calcium, endonucleases, and protease. The present study, which aims to investigate whether (1) cerulein induces apoptotic gene expression (bax, bid, p53) in pancreatic acinar AR42J cells and (2) cerulein-induced gene expression is mediated by intracellular Ca2+, monitored the gene expression profile in the cells treated with the Ca2+ chelator BAPTA-AM. Results showed that cerulein (10−7 M) evoked an initial peak Ca2+ signal; a further Ca2+ signal was induced with second treatment of cerulein. Cerulein-induced Ca2+ signal could not be detected in the cells treated with the Ca2+ chelator BAPTA-AM. Cerulein dose-dependently induced apoptosis, determined by DNA fragmentation and pro-apoptotic bid expression in AR42J cells. Cerulein induced bid, bax, and p53 mRNA expression, which was inhibited in the cells treated with cerulein and cultured in the presence of BAPTA-AM. The present results suggest that increase in the free cytosolic Ca2+ may be the upstream event of apoptotic gene (bax, bid, p53) expression, which contribute to cerulein-induced apoptosis in pancreatic acinar cells.
Full Text
http://onlinelibrary.wiley.com/doi/10.1196/annals.1299.009/abstract
DOI
OAK-2003-00048
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pharmacology (약리학교실) > 1. Journal Papers
Yonsei Authors
Kim, Kyung Hwan(김경환)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/113208
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