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A novel uracil‐DNA glycosylase family related to the helix–hairpin–helix DNA glycosylase superfamily

Authors
 Ji Hyung Chung  ;  Eun Kyoung Im  ;  Yangsoo Jang  ;  Jong Ho Lee  ;  Ki‐Chul Hwang  ;  Jaewon Oh  ;  Seahyoung Lee  ;  Jun Hye Kwon  ;  Hyun‐Young Park 
Citation
 NUCLEIC ACIDS RESEARCH, Vol.31(8) : 2045-2055, 2003 
Journal Title
 NUCLEIC ACIDS RESEARCH 
ISSN
 0305-1048 
Issue Date
2003
MeSH
Amino Acid Sequence ; Archaeal Proteins/genetics ; Archaeal Proteins/isolation & purification ; Archaeal Proteins/metabolism ; DNA Glycosylases* ; DNA Repair ; Escherichia coli/genetics ; Guanosine/analogs & derivatives* ; Guanosine/metabolism ; Methanococcus/enzymology ; Methanococcus/genetics ; Molecular Sequence Data ; N-Glycosyl Hydrolases/genetics ; N-Glycosyl Hydrolases/isolation & purification ; N-Glycosyl Hydrolases/metabolism* ; Oligonucleotides/metabolism ; Phylogeny ; Recombinant Proteins/genetics ; Recombinant Proteins/metabolism ; Sequence Homology, Amino Acid ; Substrate Specificity ; Uracil-DNA Glycosidase
Keywords
12682355
Abstract
Cytosine bases can be deaminated spontaneously to uracil, causing DNA damage. Uracil-DNA glycosylase (UDG), a ubiquitous uracil-excising enzyme found in bacteria and eukaryotes, is one of the enzymes that repair this kind of DNA damage. To date, no UDG-coding gene has been identified in Methanococcus jannaschii, although its entire genome was deciphered. Here, we have identified and characterized a novel UDG from M.jannaschii designated as MjUDG. It efficiently removed uracil from both single- and double-stranded DNA. MjUDG also catalyzes the excision of 8-oxoguanine from DNA. MjUDG has a helix-hairpin-helix motif and a [4Fe-4S]-binding cluster that is considered to be important for the DNA binding and catalytic activity. Although MjUDG shares these features with other structural families such as endonuclease III and mismatch-specific DNA glycosylase (MIG), unique conserved amino acids and substrate specificity distinguish MjUDG from other families. Also, a homologous member of MjUDG was identified in Aquifex aeolicus. We report that MjUDG belongs to a novel UDG family that has not been described to date.
Files in This Item:
T200300838.pdf Download
DOI
OAK-2003-00033
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
Yonsei Authors
Oh, Jae Won(오재원) ORCID logo https://orcid.org/0000-0002-4585-1488
Jang, Yang Soo(장양수) ORCID logo https://orcid.org/0000-0002-2169-3112
Hwang, Ki Chul(황기철)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/113193
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