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A novel uracil‐DNA glycosylase family related to the helix–hairpin–helix DNA glycosylase superfamily

DC FieldValueLanguage
dc.contributor.author오재원-
dc.contributor.author장양수-
dc.contributor.author황기철-
dc.date.accessioned2015-07-15T16:35:09Z-
dc.date.available2015-07-15T16:35:09Z-
dc.date.issued2003-
dc.identifier.issn0305-1048-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/113193-
dc.description.abstractCytosine bases can be deaminated spontaneously to uracil, causing DNA damage. Uracil-DNA glycosylase (UDG), a ubiquitous uracil-excising enzyme found in bacteria and eukaryotes, is one of the enzymes that repair this kind of DNA damage. To date, no UDG-coding gene has been identified in Methanococcus jannaschii, although its entire genome was deciphered. Here, we have identified and characterized a novel UDG from M.jannaschii designated as MjUDG. It efficiently removed uracil from both single- and double-stranded DNA. MjUDG also catalyzes the excision of 8-oxoguanine from DNA. MjUDG has a helix-hairpin-helix motif and a [4Fe-4S]-binding cluster that is considered to be important for the DNA binding and catalytic activity. Although MjUDG shares these features with other structural families such as endonuclease III and mismatch-specific DNA glycosylase (MIG), unique conserved amino acids and substrate specificity distinguish MjUDG from other families. Also, a homologous member of MjUDG was identified in Aquifex aeolicus. We report that MjUDG belongs to a novel UDG family that has not been described to date.-
dc.description.statementOfResponsibilityopen-
dc.format.extent2045~2055-
dc.relation.isPartOfNUCLEIC ACIDS RESEARCH-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAmino Acid Sequence-
dc.subject.MESHArchaeal Proteins/genetics-
dc.subject.MESHArchaeal Proteins/isolation & purification-
dc.subject.MESHArchaeal Proteins/metabolism-
dc.subject.MESHDNAGlycosylases*-
dc.subject.MESHDNARepair-
dc.subject.MESHEscherichia coli/genetics-
dc.subject.MESHGuanosine/analogs & derivatives*-
dc.subject.MESHGuanosine/metabolism-
dc.subject.MESHMethanococcus/enzymology-
dc.subject.MESHMethanococcus/genetics-
dc.subject.MESHMolecular Sequence Data-
dc.subject.MESHN-Glycosyl Hydrolases/genetics-
dc.subject.MESHN-Glycosyl Hydrolases/isolation & purification-
dc.subject.MESHN-Glycosyl Hydrolases/metabolism*-
dc.subject.MESHOligonucleotides/metabolism-
dc.subject.MESHPhylogeny-
dc.subject.MESHRecombinant Proteins/genetics-
dc.subject.MESHRecombinant Proteins/metabolism-
dc.subject.MESHSequence Homology, Amino Acid-
dc.subject.MESHSubstrate Specificity-
dc.subject.MESHUracil-DNAGlycosidase-
dc.titleA novel uracil‐DNA glycosylase family related to the helix–hairpin–helix DNA glycosylase superfamily-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Internal Medicine (내과학)-
dc.contributor.googleauthorJi Hyung Chung-
dc.contributor.googleauthorEun Kyoung Im-
dc.contributor.googleauthorYangsoo Jang-
dc.contributor.googleauthorJong Ho Lee-
dc.contributor.googleauthorKi‐Chul Hwang-
dc.contributor.googleauthorJaewon Oh-
dc.contributor.googleauthorSeahyoung Lee-
dc.contributor.googleauthorJun Hye Kwon-
dc.contributor.googleauthorHyun‐Young Park-
dc.identifier.doiOAK-2003-00033-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA03448-
dc.contributor.localIdA04456-
dc.contributor.localIdA02395-
dc.relation.journalcodeJ02387-
dc.identifier.eissn1362-4962-
dc.identifier.pmid12682355-
dc.subject.keyword12682355-
dc.contributor.alternativeNameOh, Jae Won-
dc.contributor.alternativeNameJang, Yang Soo-
dc.contributor.alternativeNameHwang, Ki Chul-
dc.contributor.affiliatedAuthorJang, Yang Soo-
dc.contributor.affiliatedAuthorHwang, Ki Chul-
dc.contributor.affiliatedAuthorOh, Jae Won-
dc.rights.accessRightsfree-
dc.citation.volume31-
dc.citation.number8-
dc.citation.startPage2045-
dc.citation.endPage2055-
dc.identifier.bibliographicCitationNUCLEIC ACIDS RESEARCH, Vol.31(8) : 2045-2055, 2003-
dc.identifier.rimsid54196-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers

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