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A novel uracil‐DNA glycosylase family related to the helix–hairpin–helix DNA glycosylase superfamily
DC Field | Value | Language |
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dc.contributor.author | 오재원 | - |
dc.contributor.author | 장양수 | - |
dc.contributor.author | 황기철 | - |
dc.date.accessioned | 2015-07-15T16:35:09Z | - |
dc.date.available | 2015-07-15T16:35:09Z | - |
dc.date.issued | 2003 | - |
dc.identifier.issn | 0305-1048 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/113193 | - |
dc.description.abstract | Cytosine bases can be deaminated spontaneously to uracil, causing DNA damage. Uracil-DNA glycosylase (UDG), a ubiquitous uracil-excising enzyme found in bacteria and eukaryotes, is one of the enzymes that repair this kind of DNA damage. To date, no UDG-coding gene has been identified in Methanococcus jannaschii, although its entire genome was deciphered. Here, we have identified and characterized a novel UDG from M.jannaschii designated as MjUDG. It efficiently removed uracil from both single- and double-stranded DNA. MjUDG also catalyzes the excision of 8-oxoguanine from DNA. MjUDG has a helix-hairpin-helix motif and a [4Fe-4S]-binding cluster that is considered to be important for the DNA binding and catalytic activity. Although MjUDG shares these features with other structural families such as endonuclease III and mismatch-specific DNA glycosylase (MIG), unique conserved amino acids and substrate specificity distinguish MjUDG from other families. Also, a homologous member of MjUDG was identified in Aquifex aeolicus. We report that MjUDG belongs to a novel UDG family that has not been described to date. | - |
dc.description.statementOfResponsibility | open | - |
dc.format | application/pdf | - |
dc.relation.isPartOf | NUCLEIC ACIDS RESEARCH | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Amino Acid Sequence | - |
dc.subject.MESH | Archaeal Proteins/genetics | - |
dc.subject.MESH | Archaeal Proteins/isolation & purification | - |
dc.subject.MESH | Archaeal Proteins/metabolism | - |
dc.subject.MESH | DNAGlycosylases* | - |
dc.subject.MESH | DNARepair | - |
dc.subject.MESH | Escherichia coli/genetics | - |
dc.subject.MESH | Guanosine/analogs & derivatives* | - |
dc.subject.MESH | Guanosine/metabolism | - |
dc.subject.MESH | Methanococcus/enzymology | - |
dc.subject.MESH | Methanococcus/genetics | - |
dc.subject.MESH | Molecular Sequence Data | - |
dc.subject.MESH | N-Glycosyl Hydrolases/genetics | - |
dc.subject.MESH | N-Glycosyl Hydrolases/isolation & purification | - |
dc.subject.MESH | N-Glycosyl Hydrolases/metabolism* | - |
dc.subject.MESH | Oligonucleotides/metabolism | - |
dc.subject.MESH | Phylogeny | - |
dc.subject.MESH | Recombinant Proteins/genetics | - |
dc.subject.MESH | Recombinant Proteins/metabolism | - |
dc.subject.MESH | Sequence Homology, Amino Acid | - |
dc.subject.MESH | Substrate Specificity | - |
dc.subject.MESH | Uracil-DNAGlycosidase | - |
dc.title | A novel uracil‐DNA glycosylase family related to the helix–hairpin–helix DNA glycosylase superfamily | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학) | - |
dc.contributor.googleauthor | Ji Hyung Chung | - |
dc.contributor.googleauthor | Eun Kyoung Im | - |
dc.contributor.googleauthor | Yangsoo Jang | - |
dc.contributor.googleauthor | Jong Ho Lee | - |
dc.contributor.googleauthor | Ki‐Chul Hwang | - |
dc.contributor.googleauthor | Jaewon Oh | - |
dc.contributor.googleauthor | Seahyoung Lee | - |
dc.contributor.googleauthor | Jun Hye Kwon | - |
dc.contributor.googleauthor | Hyun‐Young Park | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A03448 | - |
dc.contributor.localId | A04456 | - |
dc.contributor.localId | A02395 | - |
dc.relation.journalcode | J02387 | - |
dc.identifier.eissn | 1362-4962 | - |
dc.identifier.pmid | 12682355 | - |
dc.subject.keyword | 12682355 | - |
dc.contributor.alternativeName | Oh, Jae Won | - |
dc.contributor.alternativeName | Jang, Yang Soo | - |
dc.contributor.alternativeName | Hwang, Ki Chul | - |
dc.contributor.affiliatedAuthor | Jang, Yang Soo | - |
dc.contributor.affiliatedAuthor | Hwang, Ki Chul | - |
dc.contributor.affiliatedAuthor | Oh, Jae Won | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 31 | - |
dc.citation.number | 8 | - |
dc.citation.startPage | 2045 | - |
dc.citation.endPage | 2055 | - |
dc.identifier.bibliographicCitation | NUCLEIC ACIDS RESEARCH, Vol.31(8) : 2045-2055, 2003 | - |
dc.identifier.rimsid | 54196 | - |
dc.type.rims | ART | - |
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