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근세포 분화에 따른 Bcl-2와 Bcl-X_L_ 발현

Other Titles
 The Expression of Bcl-2 and Bcl-XL in Myoblast During Myogenesis 
 오승헌  ;  김원주  ;  최영철 
 Journal of the Korean Society for Clinical Neurophysiology (대한임상신경생리학회지), Vol.6(2) : 80-84, 2004 
Journal Title
Journal of the Korean Society for Clinical Neurophysiology(대한임상신경생리학회지)
Issue Date
Background: Bcl-2 and Bcl-XL has been known to be members of Bcl-2 family for regulation of long-term cell survival. We investigated the expression of Bcl-2 and Bcl-XL in myblast during myogenesis. Methods: Cultured myoblasts from the C2C12 mouse cell line were isolated at the different stages of myoblast and myotubes. The mRNA levels for Bcl-2 and Bcl-XL were determined by reverse-transcriptase PCR (RT-PCR). The immunocytochemical staining using the antibodies for Bcl-2 and Bcl-XL was performed to examine the expression of Bcl-2 and Bcl-XL in cultured C2C12 cells. Results: By immunocytochemistry, Bcl-2 was strong expressed in cultured myoblasts compared with myotubes. The expression of Bcl-XL were observed at similar degree in both myoblasts and myotubes. The RT-PCR assay showed that C2C12 cells expressed both Bcl-2 and Bcl-XL mRNA level with different pattern of expression at differential stage. The level of expression of Bcl-2 mRNA was observed most highly at early stage of myoblasts, but diminished as differentiation progressed. However, the level of expression of Bcl-XL didn’t changed during myoblast differentiation. C o n c l u s i o n s: Bcl-2 was mainly expressed myoblastic differentiation at the early stage, whereas Bcl-XL w a s expressed during whole stages of myoblastic differentiation. These findings suggest Bcl-2 is one of marker of myoblast differentiation and may play a role in normal differentiation of skeletal muscle.
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Neurology (신경과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Won Joo(김원주) ORCID logo https://orcid.org/0000-0002-5850-010X
Choi, Young Chul(최영철) ORCID logo https://orcid.org/0000-0001-5525-6861
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