Cited 38 times in
Membrane-specific expression of functional purinergic receptors in normal human nasal epithelial cells
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 최재영 | - |
dc.contributor.author | 김성식 | - |
dc.contributor.author | 김진영 | - |
dc.contributor.author | 김창훈 | - |
dc.contributor.author | 신지현 | - |
dc.contributor.author | 윤주헌 | - |
dc.contributor.author | 이민구 | - |
dc.contributor.author | 이정권 | - |
dc.date.accessioned | 2015-07-14T17:29:36Z | - |
dc.date.available | 2015-07-14T17:29:36Z | - |
dc.date.issued | 2004 | - |
dc.identifier.issn | 1040-0605 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/112993 | - |
dc.description.abstract | Extracellular purines and pyrimidines regulate various physiological responses via the cell surface receptors known as purinoreceptors and may exert autocrine or paracrine effects on ion transport, fluid transport, ciliary beat frequency, and mucin secretion. Therefore, this study aims to investigate the expression patterns of the purinoreceptors in normal human nasal epithelial (NHNE) cells. In RT-PCR, the mRNAs for several P2X (P2X3, P2X4, P2X7) and P2Y (P2Y1, P2Y2, P2Y4, P2Y6, P2Y11, P2Y12) receptors were identified in NHNE cells. Functional localizations of P2 receptors were investigated by measuring intracellular calcium concentration ([Ca2+]i) increases in membrane-specific manner using a double-perfusion chamber. Absence of the responses of αβ-methylene ATP and 2-methylthio-ATP excluded functionally active P2X3, P2X4, and P2Y1 receptors as far as [Ca2+]i increase is concerned. Applications with ATP and UTP revealed that luminal membranes of NHNE cells express P2Y2 and P2Y6 receptors and basolateral membranes express P2Y2 receptor. Expressions of P2Y2 and P2Y6 receptors in NHNE cells were further verified by immunoblotting using specific antibodies. In addition, the results with 2,3-O-(4-benzoyl)-benzoyl-ATP indicate that the P2Y11 receptor may be present on the luminal side. In conclusion, the NHNE cells express functionally active P2Y2, P2Y6, and P2Y11 receptors in a membrane-specific pattern, which may play an important role in the control of mucin and fluid secretion in NHNE cells. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 835~842 | - |
dc.relation.isPartOf | AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Base Sequence | - |
dc.subject.MESH | Calcium Signaling/physiology | - |
dc.subject.MESH | Cell Membrane/physiology* | - |
dc.subject.MESH | DNA Primers | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Kinetics | - |
dc.subject.MESH | Molecular Sequence Data | - |
dc.subject.MESH | Nasal Mucosa/physiology* | - |
dc.subject.MESH | RNA, Messenger/genetics | - |
dc.subject.MESH | Receptors, Purinergic/genetics* | - |
dc.subject.MESH | Reference Values | - |
dc.subject.MESH | Reverse Transcriptase Polymerase Chain Reaction | - |
dc.title | Membrane-specific expression of functional purinergic receptors in normal human nasal epithelial cells | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Yonsei Biomedical Research Center (연세의생명연구원) | - |
dc.contributor.googleauthor | Chang-Hoon Kim | - |
dc.contributor.googleauthor | Sung-Shik Kim | - |
dc.contributor.googleauthor | Joo-Heon Yoon | - |
dc.contributor.googleauthor | Min Goo Lee | - |
dc.contributor.googleauthor | Jeung-Gweon Lee | - |
dc.contributor.googleauthor | Wan Namkung | - |
dc.contributor.googleauthor | Jin Young Kim | - |
dc.contributor.googleauthor | Ji-Hyun Shin | - |
dc.contributor.googleauthor | Jae Young Choi | - |
dc.identifier.doi | 10.1152/ajplung.00285.2003 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A04173 | - |
dc.contributor.localId | A01050 | - |
dc.contributor.localId | A01025 | - |
dc.contributor.localId | A00574 | - |
dc.contributor.localId | A02163 | - |
dc.contributor.localId | A02604 | - |
dc.contributor.localId | A02781 | - |
dc.contributor.localId | A03095 | - |
dc.relation.journalcode | J00106 | - |
dc.identifier.eissn | 1522-1504 | - |
dc.identifier.pmid | 15208093 | - |
dc.contributor.alternativeName | Choi, Jae Young | - |
dc.contributor.alternativeName | Kim, Sung Shik | - |
dc.contributor.alternativeName | Kim, Jin Young | - |
dc.contributor.alternativeName | Kim, Chang Hoon | - |
dc.contributor.alternativeName | Shin, Ji Hyun | - |
dc.contributor.alternativeName | Yoon, Joo Heon | - |
dc.contributor.alternativeName | Lee, Min Goo | - |
dc.contributor.alternativeName | Lee, Jeung Gweon | - |
dc.contributor.affiliatedAuthor | Choi, Jae Young | - |
dc.contributor.affiliatedAuthor | Kim, Chang Hoon | - |
dc.contributor.affiliatedAuthor | Kim, Jin Young | - |
dc.contributor.affiliatedAuthor | Kim, Sung Shik | - |
dc.contributor.affiliatedAuthor | Shin, Ji Hyun | - |
dc.contributor.affiliatedAuthor | Yoon, Joo Heon | - |
dc.contributor.affiliatedAuthor | Lee, Min Goo | - |
dc.contributor.affiliatedAuthor | Lee, Jeung Gweon | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 287 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 835 | - |
dc.citation.endPage | 842 | - |
dc.identifier.bibliographicCitation | AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY, Vol.287(4) : 835-842, 2004 | - |
dc.identifier.rimsid | 36843 | - |
dc.type.rims | ART | - |
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