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Allergenic characterization of tropomyosin from the dusky brown cockroach, Periplaneta fuliginosa

Authors
 Kyoung Yong Jeong  ;  Heeyu Hwang  ;  Tai-Soon Yong  ;  Han-Il Ree  ;  Chein-Soo Hong  ;  Dong Soo Kim  ;  In-Yong Lee  ;  Jongweon Lee 
Citation
 CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY , Vol.11(4) : 680-685, 2004 
Journal Title
 CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY 
ISSN
 1071-412X 
Issue Date
2004
MeSH
Adolescent ; Adult ; Amino Acid Sequence ; Animals ; Base Sequence ; Child ; Cloning, Molecular ; Cross Reactions ; DNA, Complementary/analysis ; Electrophoresis, Polyacrylamide Gel ; Enzyme-Linked Immunosorbent Assay ; Female ; Humans ; Immunoblotting ; Immunoglobulin E/immunology* ; Male ; Middle Aged ; Molecular Sequence Data ; Periplaneta/genetics ; Periplaneta/immunology* ; Recombinant Proteins/genetics ; Recombinant Proteins/immunology* ; Reverse Transcriptase Polymerase Chain Reaction ; Sequence Homology ; Species Specificity ; Tropomyosin/genetics* ; Tropomyosin/immunology*
Abstract
Household arthropods are one of the most common causes of allergic diseases. Four species of cockroaches are found to reside in Korean homes, but published work deals almost exclusively with the German and American cockroaches. This study was undertaken to investigate the cross-reactive allergenic components of the dusky brown cockroach, Periplaneta fuliginosa. Enzyme-linked immunosorbent assay (ELISA) inhibition and immunoblot analyses for the dusky brown cockroach were performed with Blattella germanica and Dermatophagoides farinae allergic sera. cDNA encoding tropomyosin, which is a well known cross-reactive pan-allergen, was cloned by reverse transcriptase PCR, and recombinant protein was produced by using a pET-28b expression system. Native tropomyosin was purified by ammonium sulfate fractionation and electroelution. The immunoglobulin E (IgE) reactivities of native and recombinant tropomyosins were compared by an ELISA inhibition study. All 30 sera tested showed P. fuliginosa-specific IgE, and the IgE-binding reactivity of the P. fuliginosa extract was inhibited as much as 79.4% by a B. germanica extract and as much as 63.3% by a D. farinae extract. The deduced amino acid sequence of cloned cDNA was identical with that of Periplaneta americana tropomyosin (98.5% nucleotide sequence identity). Seven of 26 (26.9%) allergic sera had IgE specific for recombinant protein, and the maximum inhibition of P. fuliginosa-specific IgE achieved with recombinant tropomyosin was 37.7% at an inhibitor concentration of 10 μg/ml. Native tropomyosin inhibited the binding of IgE to the P. fuliginosa, B. germanica, and D. farinae extracts by 65.0, 51.8, and 39% at an inhibitor concentration of 1 μg/ml. P. fuliginosa appears to possess allergens that are highly cross-reactive with allergens of B. germanica and D. farinae. Tropomyosin was found to be a major allergenic component accounting for the cross-reactivity between cockroaches and dust mites.
Files in This Item:
T200403988.pdf Download
DOI
10.1128/CDLI.11.4.680-685.2004
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Environmental Medical Biology (환경의생물학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pediatrics (소아청소년과학교실) > 1. Journal Papers
5. Research Institutes (연구소) > Institute of Allergy (알레르기연구소) > 1. Journal Papers
Yonsei Authors
Kim, Dong Soo(김동수)
Yong, Tai Soon(용태순) ORCID logo https://orcid.org/0000-0002-3445-0769
Lee, In Yong(이인용)
Lee, Jong Weon(이종원)
Ree, Han Il(이한일)
Jeong, Kyoung Yong(정경용) ORCID logo https://orcid.org/0000-0001-9887-1426
Hong, Chein Soo(홍천수)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/112877
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