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Molecular Cloning and Characterization of Tropomyosin, a Major Allergen of Chironomus kiiensis, a Dominant Species of Nonbiting Midges in Korea

Authors
 Kyoung Yong Jeong  ;  Hye-Yung Yum  ;  Tai-Soon Yong  ;  Dong Soo Kim  ;  Chein-Soo Hong  ;  Han-Il Ree  ;  In-Yong Lee 
Citation
 CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY , Vol.11(2) : 320-324, 2004 
Journal Title
 CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY 
ISSN
 1071-412X 
Issue Date
2004
MeSH
Allergens/genetics ; Allergens/immunology ; Amino Acid Sequence ; Animals ; Base Sequence ; Chironomidae/immunology* ; Cloning, Molecular ; Gene Library ; Humans ; Hypersensitivity/immunology* ; Immunoblotting ; Immunoglobulin E/blood ; Korea ; Mice ; Mice, Inbred BALB C ; Molecular Sequence Data ; Recombinant Proteins/genetics ; Recombinant Proteins/immunology ; Recombinant Proteins/isolation & purification ; Tropomyosin/genetics* ; Tropomyosin/immunology*
Abstract
Chironomids are widely and abundantly distributed in the vicinity of standing waters. Larvae of Chironomus and some other genera are known to contain hemoglobins, which have been described as a major allergen, and the adults that have no hemoglobins also have been reported to contain allergens. In this study, we tried to establish the role of chironomid allergy and characterize the allergen of Chironomus kiiensis adults. Skin tests using C. kiiensis adult extracts were performed on patients with allergic symptoms. A cDNA library of C. kiiensis adults was screened with C. kiiensis immune mouse sera to identify allergens, and results were confirmed using skin test-positive human sera. Recombinant allergen was expressed in Escherichia coli and purified by affinity chromatography using nickel-nitrilotriacetic acid agarose to investigate its allergenic properties. Out of 275 allergic patients 14.2% showed a positive reaction to C. kiiensis adult crude extracts in the skin test. The tropomyosin was cloned by immunoscreening and expressed in Escherichia coli. C. kiiensis tropomyosin has a high homology at the amino acid level with tropomyosins which were previously known to be allergens in various arthropods (Periplaneta americana, 86.3%; Panulirus stimpson, 78.9%; Dermatophagoides pteronyssinus, 76.5%). Specific immunoglobulin E antibodies reacting to recombinant tropomyosin were detected in 17 (81%) of 21 patients whose skin test results were positive. Cross-reactivity against house dust mites and other insects was noticed with mouse anti-recombinant tropomyosin immune serum. C. kiiensis adults were shown to be an important source of inhalant allergens in Korea. Molecular cloning of C. kiiensis tropomyosin was performed and IgE reactivity was demonstrated using skin test-positive human sera. Recombinant tropomyosin will be useful for further studies or clinical applications.
Files in This Item:
T200403987.pdf Download
DOI
10.1128/CDLI.11.2.320-324.2004
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pediatrics (소아청소년과학교실) > 1. Journal Papers
5. Research Institutes (연구소) > Institute of Allergy (알레르기연구소) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Environmental Medical Biology (환경의생물학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Dong Soo(김동수)
Yong, Tai Soon(용태순) ORCID logo https://orcid.org/0000-0002-3445-0769
Jeong, Kyoung Yong(정경용) ORCID logo https://orcid.org/0000-0001-9887-1426
Hong, Chein Soo(홍천수)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/112874
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