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Multiple Polymorphic Loci for Molecular Typing of Strains of Mycobacterium leprae

 Nathan A. Groathouse  ;  Becky Rivoire  ;  Varalakshmi D. Vissa  ;  Patrick J. Brennan  ;  Sang-Nae Cho  ;  Hyeyoung Lee  ;  Hansuk Kim 
 JOURNAL OF CLINICAL MICROBIOLOGY, Vol.42(4) : 1666-1672, 2004 
Journal Title
Issue Date
Animals ; Armadillos/microbiology ; Bacterial Typing Techniques/methods* ; Humans ; Leprosy/microbiology ; Minisatellite Repeats/genetics* ; Mycobacterium leprae/classification* ; Mycobacterium leprae/genetics ; Polymerase Chain Reaction ; Polymorphism, Genetic* ; Polymorphism, Restriction Fragment Length ; Sequence Analysis, DNA
The need for molecular tools for the differentiation of isolates of Mycobacterium leprae, the organism that causes leprosy, is urgent in view of the continuing high levels of new case detection, despite years of aggressive chemotherapy and the consequent reduction in the prevalence of leprosy. The slow onset of leprosy and the reliance on physical examination for detection of disease have restricted the epidemiological tracking necessary to understand and control transmission. Two genetic loci in several isolates of M. leprae have previously been demonstrated to contain variable-number tandem repeats (VNTRs). On the basis of these reports and the availability of the full genome sequence, multiple-locus VNTR analysis for strain typing has been undertaken. A panel of 11 short tandem repeat (STR) loci with repeat units of 1, 2, 3, 6, 12, 18, 21, and 27 bp from four clinical isolates of M. leprae propagated in armadillo hosts were screened by PCR. Fragment length polymorphisms were detected at 9 of the 11 loci by agarose gel electrophoresis. Sequencing of representative DNA products confirmed the presence of VNTRs between isolates. The application of nine new polymorphic STRs in conjunction with automated methods for electrophoresis and size determination allows greater discrimination between isolates of M. leprae and enhances the potential of this technique to track the transmission of leprosy.
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1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers
Yonsei Authors
Cho, Sang Nae(조상래)
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