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CCAAT/Enhancer Binding Protein and Nuclear Factor-Y Regulate Adiponectin Gene Expression in Adipose Tissue

 Sang-kyu Park  ;  So-Young Oh  ;  Jae-woo Kim  ;  Kyung-Sup Kim  ;  Sarah Yoon  ;  Min-Young Lee 
 DIABETES, Vol.53(11) : 2757-2766, 2004 
Journal Title
Issue Date
Adipocytes/cytology ; Adipocytes/physiology* ; Adiponectin ; Animals ; Base Sequence ; Binding Sites ; CCAAT-Binding Factor/metabolism* ; CCAAT-Enhancer-Binding Proteins/metabolism* ; Cell Differentiation ; Cell Nucleus/genetics ; Cell Nucleus/physiology ; Deoxyribonuclease I ; Gene Expression Regulation* ; Humans ; Intercellular Signaling Peptides and Proteins/genetics* ; Mice ; Molecular Sequence Data ; Promoter Regions, Genetic ; RNA, Messenger/genetics ; Reverse Transcriptase Polymerase Chain Reaction ; Sequence Alignment ; Sequence Homology, Nucleic Acid ; Transcription Factors/metabolism*
Adiponectin is one of the adipokines secreted by adipocytes and regulates energy homeostasis associated with insulin sensitivity, suggesting a possibility of nutritional regulation of adiponectin gene expression. In this study, we showed that the transcription of adiponectin gene was induced 4–6 h after refeeding of mice. Also, differentiated 3T3-L1 adipocytes that were treated with high glucose expressed significantly increased adiponectin mRNA. Promoter analysis using nuclear extracts from white adipose tissue revealed that CCAAT/enhancer binding protein (C/EBP) and nuclear factor-Y (NF-Y) bound on the −117/−73 region of the adiponectin promoter. This region was critical for the activity of the adiponectin promoter as the deletion or mutation of this region markedly diminished the promoter activity to a basal level. Furthermore, the C/EBP binding increased in both refed animal and high glucose-treated 3T3-L1 adipocytes in an electrophoretic mobility shift assay, suggesting that C/EBP is responsible for the dietary response of the adiponectin gene expression. Chromatin immunoprecipitation studies demonstrated the binding of C/EBP and NF-Y in both mouse and differentiated 3T3-L1 adipocytes and also that C/EBP binding increased in response to high glucose. These findings demonstrated that C/EBP and NF-Y are critical for the regulation of the adiponectin expression in response to nutrients and in the course of adipocyte differentiation.
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1. College of Medicine (의과대학) > Dept. of Biochemistry and Molecular Biology (생화학-분자생물학교실) > 1. Journal Papers
Yonsei Authors
Kim, Kyung Sup(김경섭) ORCID logo https://orcid.org/0000-0001-8483-8537
Kim, Jae Woo(김재우) ORCID logo https://orcid.org/0000-0001-5456-9495
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