Cited 89 times in
CCAAT/Enhancer Binding Protein and Nuclear Factor-Y Regulate Adiponectin Gene Expression in Adipose Tissue
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김경섭 | - |
dc.contributor.author | 김재우 | - |
dc.date.accessioned | 2015-07-14T17:23:29Z | - |
dc.date.available | 2015-07-14T17:23:29Z | - |
dc.date.issued | 2004 | - |
dc.identifier.issn | 0012-1797 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/112787 | - |
dc.description.abstract | Adiponectin is one of the adipokines secreted by adipocytes and regulates energy homeostasis associated with insulin sensitivity, suggesting a possibility of nutritional regulation of adiponectin gene expression. In this study, we showed that the transcription of adiponectin gene was induced 4–6 h after refeeding of mice. Also, differentiated 3T3-L1 adipocytes that were treated with high glucose expressed significantly increased adiponectin mRNA. Promoter analysis using nuclear extracts from white adipose tissue revealed that CCAAT/enhancer binding protein (C/EBP) and nuclear factor-Y (NF-Y) bound on the −117/−73 region of the adiponectin promoter. This region was critical for the activity of the adiponectin promoter as the deletion or mutation of this region markedly diminished the promoter activity to a basal level. Furthermore, the C/EBP binding increased in both refed animal and high glucose-treated 3T3-L1 adipocytes in an electrophoretic mobility shift assay, suggesting that C/EBP is responsible for the dietary response of the adiponectin gene expression. Chromatin immunoprecipitation studies demonstrated the binding of C/EBP and NF-Y in both mouse and differentiated 3T3-L1 adipocytes and also that C/EBP binding increased in response to high glucose. These findings demonstrated that C/EBP and NF-Y are critical for the regulation of the adiponectin expression in response to nutrients and in the course of adipocyte differentiation. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 2757~2766 | - |
dc.relation.isPartOf | DIABETES | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Adipocytes/cytology | - |
dc.subject.MESH | Adipocytes/physiology* | - |
dc.subject.MESH | Adiponectin | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Base Sequence | - |
dc.subject.MESH | Binding Sites | - |
dc.subject.MESH | CCAAT-Binding Factor/metabolism* | - |
dc.subject.MESH | CCAAT-Enhancer-Binding Proteins/metabolism* | - |
dc.subject.MESH | Cell Differentiation | - |
dc.subject.MESH | Cell Nucleus/genetics | - |
dc.subject.MESH | Cell Nucleus/physiology | - |
dc.subject.MESH | Deoxyribonuclease I | - |
dc.subject.MESH | Gene Expression Regulation* | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Intercellular Signaling Peptides and Proteins/genetics* | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Molecular Sequence Data | - |
dc.subject.MESH | Promoter Regions, Genetic | - |
dc.subject.MESH | RNA, Messenger/genetics | - |
dc.subject.MESH | Reverse Transcriptase Polymerase Chain Reaction | - |
dc.subject.MESH | Sequence Alignment | - |
dc.subject.MESH | Sequence Homology, Nucleic Acid | - |
dc.subject.MESH | Transcription Factors/metabolism* | - |
dc.title | CCAAT/Enhancer Binding Protein and Nuclear Factor-Y Regulate Adiponectin Gene Expression in Adipose Tissue | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Biochemistry & Molecular Biology (생화학,분자생물학) | - |
dc.contributor.googleauthor | Sang-kyu Park | - |
dc.contributor.googleauthor | So-Young Oh | - |
dc.contributor.googleauthor | Jae-woo Kim | - |
dc.contributor.googleauthor | Kyung-Sup Kim | - |
dc.contributor.googleauthor | Sarah Yoon | - |
dc.contributor.googleauthor | Min-Young Lee | - |
dc.identifier.doi | 10.2337/diabetes.53.11.2757 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00297 | - |
dc.contributor.localId | A00865 | - |
dc.relation.journalcode | J00718 | - |
dc.identifier.eissn | 1939-327X | - |
dc.identifier.pmid | 15504955 | - |
dc.contributor.alternativeName | Kim, Kyung Sup | - |
dc.contributor.alternativeName | Kim, Jae Woo | - |
dc.contributor.affiliatedAuthor | Kim, Kyung Sup | - |
dc.contributor.affiliatedAuthor | Kim, Jae Woo | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 53 | - |
dc.citation.number | 11 | - |
dc.citation.startPage | 2757 | - |
dc.citation.endPage | 2766 | - |
dc.identifier.bibliographicCitation | DIABETES, Vol.53(11) : 2757-2766, 2004 | - |
dc.identifier.rimsid | 44505 | - |
dc.type.rims | ART | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.