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배양된 구강점막 각화상피세포가 누드마우스 피부 창상 치유에 미치는 효과

Other Titles
 The inductive capacity of primary cultured oral mucosal keratinocytes in skinwound healing of athymic nude mice 
 김현실  ;  김남희  ;  차인호  ;  김진 
 Journal of the Korean Association of Oral and Maxillofacial Surgeons (대한구강악안면외과학회지), Vol.30(4) : 308-315, 2004 
Journal Title
Journal of the Korean Association of Oral and Maxillofacial Surgeons(대한구강악안면외과학회지)
Issue Date
Oral mucosal keratinocyte ; Primary culture ; Wound healing ; Tissue engineering
Aim: The aim of this study was to investigate the mechanism of promoted skin wound healing in skin defects with primary cultured oral mucosal keratinocytes. Materials and methods: Thirty adult female nude mice weighing 20+/-2g were used for the experiment. Primary cultured and suspended oral mucosal keratinocytes, labeled with BrdU, were scattered onto 1.5cm*1.5cm sized full thickness skin defects in the experimental group(N=15), and no grafts were placed the control group(N=15). They were sacrificed at 3 days, 1 week and 2 weeks after the treatment respectively. Histological examination of each wounds were performed to review the healing progress on measuring the length from the wound margin to regenerating epithelial front. The role of keratinocytes were assessed by double immunohistochemical staining with Anti-BrdU and Anti-cytokeratin AE1/3. Results: In the experimental group the wound was completely covered with regenerating epithelia in 2 weeks, but partially regenerated in the control group. The immunohistochemical studies unexpectedly reveal that most of regenerating epithelial cells were induced from marginal epithelium of the margin, not from the scattered keratinocytes. Conclusion: We could successfully confirm that graft of primary cultured oral mucosal keratinocytes promotes the regeneration of skin defects.
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Appears in Collections:
2. College of Dentistry (치과대학) > Research Institute (부설연구소) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Oral Pathology (구강병리학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Oral and Maxillofacial Surgery (구강악안면외과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Nam Hee(김남희) ORCID logo https://orcid.org/0000-0002-3087-5276
Kim, Jin(김진)
Kim, Hyun Sil(김현실) ORCID logo https://orcid.org/0000-0003-3614-1764
Cha, In Ho(차인호) ORCID logo https://orcid.org/0000-0001-8259-2190
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