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배양된 구강점막 각화상피세포가 누드마우스 피부 창상 치유에 미치는 효과

DC FieldValueLanguage
dc.contributor.author김남희-
dc.contributor.author김진-
dc.contributor.author김현실-
dc.contributor.author차인호-
dc.date.accessioned2015-07-14T17:20:40Z-
dc.date.available2015-07-14T17:20:40Z-
dc.date.issued2004-
dc.identifier.issn1225-1585-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/112692-
dc.description.abstractAim: The aim of this study was to investigate the mechanism of promoted skin wound healing in skin defects with primary cultured oral mucosal keratinocytes. Materials and methods: Thirty adult female nude mice weighing 20+/-2g were used for the experiment. Primary cultured and suspended oral mucosal keratinocytes, labeled with BrdU, were scattered onto 1.5cm*1.5cm sized full thickness skin defects in the experimental group(N=15), and no grafts were placed the control group(N=15). They were sacrificed at 3 days, 1 week and 2 weeks after the treatment respectively. Histological examination of each wounds were performed to review the healing progress on measuring the length from the wound margin to regenerating epithelial front. The role of keratinocytes were assessed by double immunohistochemical staining with Anti-BrdU and Anti-cytokeratin AE1/3. Results: In the experimental group the wound was completely covered with regenerating epithelia in 2 weeks, but partially regenerated in the control group. The immunohistochemical studies unexpectedly reveal that most of regenerating epithelial cells were induced from marginal epithelium of the margin, not from the scattered keratinocytes. Conclusion: We could successfully confirm that graft of primary cultured oral mucosal keratinocytes promotes the regeneration of skin defects.-
dc.description.statementOfResponsibilityopen-
dc.format.extent308~315-
dc.relation.isPartOfJournal of the Korean Association of Oral and Maxillofacial Surgeons (대한구강악안면외과학회지)-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.title배양된 구강점막 각화상피세포가 누드마우스 피부 창상 치유에 미치는 효과-
dc.title.alternativeThe inductive capacity of primary cultured oral mucosal keratinocytes in skinwound healing of athymic nude mice-
dc.typeArticle-
dc.contributor.collegeResearcher Institutes (부설 연구소)-
dc.contributor.departmentOral Cancer Research Institute (구강종양연구소)-
dc.contributor.googleauthor김현실-
dc.contributor.googleauthor김남희-
dc.contributor.googleauthor차인호-
dc.contributor.googleauthor김진-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA00360-
dc.contributor.localIdA01121-
dc.contributor.localIdA04002-
dc.contributor.localIdA01009-
dc.relation.journalcodeJ01806-
dc.subject.keywordOral mucosal keratinocyte-
dc.subject.keywordPrimary culture-
dc.subject.keywordWound healing-
dc.subject.keywordTissue engineering-
dc.contributor.alternativeNameKim, Nam Hee-
dc.contributor.alternativeNameKim, Jin-
dc.contributor.alternativeNameKim, Hyun Sil-
dc.contributor.alternativeNameCha, In Ho-
dc.contributor.affiliatedAuthorKim, Nam Hee-
dc.contributor.affiliatedAuthorKim, Hyun Sil-
dc.contributor.affiliatedAuthorCha, In Ho-
dc.contributor.affiliatedAuthorKim, Jin-
dc.rights.accessRightsfree-
dc.citation.volume30-
dc.citation.number4-
dc.citation.startPage308-
dc.citation.endPage315-
dc.identifier.bibliographicCitationJournal of the Korean Association of Oral and Maxillofacial Surgeons (대한구강악안면외과학회지), Vol.30(4) : 308-315, 2004-
Appears in Collections:
5. Research Institutes (연구소) > Oral Cancer Research Institute (구강종양연구소) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Oral Pathology (구강병리학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Oral and Maxillofacial Surgery (구강악안면외과학교실) > 1. Journal Papers

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