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말디도프 질량 분석을 이용한 한국인의 B형간염 바이러스 유전자형 및 임상적 의의에 대한 재평가

Other Titles
 Reappraisal of HBV Genotypes and Clinical Significance in Koreans Using MALDI-TOF Mass Spectrometry 
 이중민  ;  안상훈  ;  문영명  ;  전재윤  ;  한광협  ;  김수옥  ;  정현재  ;  홍선표  ;  심명기  ;  김도영  ;  신지은  ;  장혜영 
 Korean Journal of Hepatology (대한간학회지), Vol.10(4) : 260-270, 2004 
Journal Title
Korean Journal of Hepatology(대한간학회지)
Issue Date
Adolescent ; Adult ; Aged ; Female ; Genotype ; Hepatitis B virus/classification ; Hepatitis B virus/genetics* ; Hepatitis B, Chronic/virology ; Humans ; Korea ; Male ; Middle Aged ; Polymorphism, Restriction Fragment Length ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization*
Background: Recent studies have shown that the genotype of hepatitis B virus (HBV) may correlate with the disease natural history and treatment outcome. However, several of these studies used low sensitivity assays in a small number of patients, and this has precluded an accurate evaluation of Korean HBV genotypes. We analyzed Korean HBV genotypes in a large population by employing a new technology, restriction fragment mass polymorphism (RFMP) using MALDI-TOF mass spectrometry, in a sensitive and specific manner.

Methods: Between February 1995 and December 2003, a total of 475 patients with chronic HBV infection were enrolled. The assay is based on the mass measurement of oligonucleotides having genotypic variations of the S gene. Clinical features including the virologic status and disease progression were also evaluated.

Results: The median age of the total patients was 35.5 years. Out of 475 patients, there were 162 (34.1%) inactive carriers, 172 (36.2%) had chronic hepatitis, 77 (16.2%) had liver cirrhosis and 64 (13.5%) had hepatocellular carcinoma (HCC). There were 454 patients (95.6%) with genotype C, 4 patients (0.8%) with genotype A, 16 patients (3.4%) with the mixed A and C genotype [7 patients (1.4%) with AA], and 1 patient (0.2%) with B genotype. Comparing genotype A and C, genotype A patients were all inactive carriers without HCC, whereas genotype C patients included those with chronic hepatitis, liver cirrhosis and HCC.

Conclusions: HBV genotype C is highly prevalent in Korea. Although it is a small percentage, genotype A also exists and it seems to take a more benign clinical course than genotype C. Further studies are necessitated to assess the relationship between HBV genotypes and the various aspects of the diseases’ clinical course.
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Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
Yonsei Authors
Kim, Do Young(김도영)
Shin, Ji Eun(신지은)
Sim, Myoung Ki(심명기)
Ahn, Sang Hoon(안상훈) ORCID logo https://orcid.org/0000-0002-3629-4624
Lee, Jung Min(이중민)
Chang, Hye Young(장혜영)
Chon, Chae Yoon(전재윤)
Han, Kwang-Hyub(한광협) ORCID logo https://orcid.org/0000-0003-3960-6539
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