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Kainate 유발 간질모델에서 Apurinic/Apyrimidinic Endonuclease의 빠른 소실과 이에 따른 세포고사

Other Titles
 Rapid Loss of Apurinic/Apyrimidinic Endonuclease and Subsequent Apoptosis in Kainate-Induced Seizure Model 
 신하영  ;  이두재  ;  이병인  ;  김경환  ;  허경  ;  이용현  ;  김미애  ;  조경주 
 Journal of Korean Epilepsy Society (대한간질학회지), Vol.8(2) : 108-115, 2004 
Journal Title
Journal of Korean Epilepsy Society(대한간질학회지)
Issue Date
Kainate ; Epilepsy ; 8-hydroxyguanine ; APE ; Apoptosis
Purpose: The DNA repair enzyme, apurinic/apyrimidinic endonuclease (APE) plays a role in base excision repair pathway involved in repairing apurinic/apyrimidinic (AP) site after oxidative stress. To reveal the relationship between APE and neuronal apoptosis associated with oxidative stress after kainate treatment, the temporal change of APE expression was investigated in kainate-induced seizure model.

Methods: Status epilepticus was induced by unilateral intrahippocampal injection of kainate. Superoxide anion radical production and DNA oxidation were evaluated by in situ detection of oxidized hydroethidine and 8-hydroxyguanine (8-OHG) immunoreactivity. APE expression was examined by Western blot and immunohistochemical analysis. DNA fragmentation was visualized with terminal deoxynucleotidyl transferase-mediated uridine 5´-triphosphate-biotin nick end labeling (TUNEL) staining.

Results: Cell loss occurred at 24 hr in CA1, CA2, and CA3 after kainate-injection. 8-OHG immunoreactivity and oxidized hydroethidine were increased comparing with control after kainate-injection. APE immunoreactivity was decreased 4 and 24 hours in the hippocampus after kainate-injection. TUNEL-positive cells were observed 24 hours but not 4 hours in hippocampus after kainate-injection. In double labeling with APE and TUNEL, TUNEL-positive cells did not show APE immunoreactivity. These data showed that cellular oxidative stress was increased, thereby APE was decreased in the hippocampus after kainate-injection. Also, it was shown that the reduction of APE preceded DNA fragmentation.

Conclusion: This study suggests that rapid loss of APE may produce the failure of DNA repair-machinary and then induce neuronal apoptosis following kainate-injection.
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1. College of Medicine (의과대학) > Dept. of Neurology (신경과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Gyung Whan(김경환) ORCID logo https://orcid.org/0000-0001-7053-4372
Kim, Mi Ae(김미애)
Lee, Byung In(이병인)
Heo, Kyoung(허경)
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