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인체 기관지 상피세포에서 Mycoplasma pneumoniae 항원이 IL-8 유전자 발현에 미치는 영향

Other Titles
 Effect of Mycoplasma pneumoniae on IL-8 Gene Expression in Human Respiratory Epithelial Cells 
 손명현  ;  이경은  ;  김규언  ;  장명웅  ;  장광천  ;  김우경  ;  권병철  ;  최성연 
 Pediatric Allergy and Respiratory Disease (소아알레르기 호흡기학회지), Vol.14(1) : 38-45, 2004 
Journal Title
Pediatric Allergy and Respiratory Disease(소아알레르기 호흡기학회지)
Issue Date
Mycoplasma pneumoniae ; Interleukin-8 ; Respiratory epithelial cells
Purpose : Mycoplasma pneumoniae is a common cause of lower respiratory disease. Several studies have suggested that M. pneumoniae respiratory infection is associated with re-active airway disease and asthma. It has been suggested that Interleukin-8(IL-8) has a role in the pathogenesis of allergic inflammation of bronchial asthma and is well known to be expressed in bronchial epithelial cells.
Methods : In this study, we examined the effect of M pneumoniae extract and the role of mitogen-activated protein kinases(MAPKs) on IL-8 expression in human lung epithelial cells. IL-8 levels were determined by an enzyme linked-immunosorbent assay. Extracellular signal-regulated kinase(ERK) phosphorylation was assessed by western blotting.
Results : In A549 cells, AT. pneumoniae extract induced IL-8 release in a tame- or dose-dependent manner. Pretreatment with PD98059, which blocks the activation of MAPK/ERK kinase 1, before M. pneumoniae extract stimulation inhibited the increase in the production of IL-8 by 64.4% at 25 M. Stimulation of A549 cells by extract caused an increase in the activity of ERK, compared with the non-stimulated cells. The extract stimulation had no effect on the activities of p38.
Conclusion : These observations suggest that activation of ERK by M. pneumoniae ex-tract may be one of the mechanisms that result in the increase of the production of IL-8.
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1. College of Medicine (의과대학) > Dept. of Pediatrics (소아과학교실) > 1. Journal Papers
Yonsei Authors
Kwon, Byoung Chul(권병철)
Kim, Kyu Earn(김규언)
Sohn, Myung Hyun(손명현) ORCID logo https://orcid.org/0000-0002-2478-487X
Choi, Sung Yon(최성연)
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