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유방암 환자의 수술 중 rapid cytokeratin 면역화학 염색법을 이용한 감시림프절의 평가
DC Field | Value | Language |
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dc.contributor.author | 박병우 | - |
dc.contributor.author | 오기근 | - |
dc.contributor.author | 유영훈 | - |
dc.contributor.author | 이일균 | - |
dc.contributor.author | 이희대 | - |
dc.contributor.author | 장항석 | - |
dc.contributor.author | 정우희 | - |
dc.contributor.author | 정준 | - |
dc.contributor.author | 홍순원 | - |
dc.date.accessioned | 2015-07-14T17:04:50Z | - |
dc.date.available | 2015-07-14T17:04:50Z | - |
dc.date.issued | 2004 | - |
dc.identifier.issn | 1738-6756 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/112165 | - |
dc.description.abstract | Purpose: Sentinel lymph node (SLN) biopsy has become a new standard procedure in the treatment of patients with early breast cancer. Furthermore, many institutions have begun offering the sentinel lymph node biopsy without simultaneous axillary dissection as a possible standard procedure when the SLN was free from tumors. For appropriate intraoperative decision making on the presence of cancer cells in axillary lymph nodes, a fast and accurate method to assess the SLN is required. The authors performed a prospective investigation of the relative merits of rapid cytokeratin immunohistochemical (IHC) staining of the SLN removed during the operations of breast cancer patients. Methods: Between December 2002 and August 2003, 38 patients with T1and T2 breast cancer were enrolled after undergoing successful sentinel lymph node biopsy. A total of 60 sentinel lymph nodes (mean number, 1.58) were biopsied and first examined by hematoxylin-eosin (H&E) staining. All the tumor free sentinel lymph nodes by H&E stained section were immunostained for cytokeratin using a rapid immunohistochemical assay (Cytokeratin (PAN), 1:50,Newcastle, UK) during the operation. Finally, the sentinel lymph nodes were submitted for paraffin embedding and serial section after surgery. Both H&E stained and cytokeratin immunostained sections were also performed. Results: This technique has a turnaround time of less than 20 minutes during the operation. Rapid IHC staining revealed 4 positive sentinel lymph nodes that were negative for metastasis by H&E staining. Among these false negative 4 cases, two cases had problems with the frozen section of H&E staining and the other 2 cases had problems due to micrometastasis. This study showed a sensitivity of 88.89%, a specificity of 100%, an accuracy of 98.33%, and a negative predictive value of 98.08%. The false-negative case (1 of 52), which was negative on H&E staining and rapid IHC staining during the operation, was disclosed as positive only after a serial permanent section examination with IHC stain. Conclusion: The introperative examination of sentinel lymph nodes is a highly accurate and effective way of predicting the axillary lymph node status of patients with breast cancers. This may be a promising technique in deciding whether to spare axillary lymph node dissection for the patient in the operating room | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 154~160 | - |
dc.relation.isPartOf | Journal of Korean Breast Cancer Society (한국유방암학회지) | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | 유방암 환자의 수술 중 rapid cytokeratin 면역화학 염색법을 이용한 감시림프절의 평가 | - |
dc.title.alternative | Intraoperative Examination of Sentinel Lymph Nodes Using Rapid Cytokeratin Immunohistochemical Staining in Breast Cancer Patients | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Surgery (외과학) | - |
dc.contributor.googleauthor | 이일균 | - |
dc.contributor.googleauthor | 정준 | - |
dc.contributor.googleauthor | 이희대 | - |
dc.contributor.googleauthor | 유영훈 | - |
dc.contributor.googleauthor | 오기근 | - |
dc.contributor.googleauthor | 홍순원 | - |
dc.contributor.googleauthor | 정우희 | - |
dc.contributor.googleauthor | 박병우 | - |
dc.contributor.googleauthor | 장항석 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.relation.journalcode | J01504 | - |
dc.subject.keyword | Breast cancer | - |
dc.subject.keyword | Sentinel lymph node | - |
dc.subject.keyword | Frozen section | - |
dc.subject.keyword | Rapid immunohistochemistry | - |
dc.subject.keyword | Cytokeratin | - |
dc.subject.keyword | 유방암 | - |
dc.subject.keyword | 감시림프절 | - |
dc.subject.keyword | 동결절편검사 | - |
dc.subject.keyword | 신속면역조직화학염색 | - |
dc.contributor.alternativeName | Park, Byeong Woo | - |
dc.contributor.alternativeName | Oh, Ki Keun | - |
dc.contributor.alternativeName | Ryu, Young Hoon | - |
dc.contributor.alternativeName | Lee, Il Kyun | - |
dc.contributor.alternativeName | Lee, Hy De | - |
dc.contributor.alternativeName | Chang, Hang Seok | - |
dc.contributor.alternativeName | Jung, Woo Hee | - |
dc.contributor.alternativeName | Jeong, Joon | - |
dc.contributor.alternativeName | Hong, Soon Won | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 7 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 154 | - |
dc.citation.endPage | 160 | - |
dc.identifier.bibliographicCitation | Journal of Korean Breast Cancer Society (한국유방암학회지), Vol.7(3) : 154-160, 2004 | - |
dc.identifier.rimsid | 45412 | - |
dc.type.rims | ART | - |
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