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사람 구강암종 세포주에서 Non-Steroidal Anti-Inflammatory Drug-Activated Gene(NAG-1)의 유도 및 세포고사 작용

Other Titles
 Induction of Non-Steroidal Anti-Inflammatory Drug-Activated Gene(NAG-1) in Human Oral Cavity Cancer Cells and its Effect on Apoptosis 
 이주환  ;  윤주헌  ;  김경수  ;  이윤재  ;  장정현  ;  이정권 
 Korean Journal of Otolaryngology (대한이비인후과학회지), Vol.47(10) : 1030-1037, 2004 
Journal Title
Korean Journal of Otolaryngology(대한이비인후과학회지)
Issue Date
Non-steroidal anti-inflammatory agents ; Oral cancers ; Apoptosis
Background and Objectives:Nonsteroidal anti-inflammatory drug (NSAID)-activated gene (NAG-1), which is induced by NSAIDs, has proapoptotic and antitumorigenic effects in colorectal cancer cells. However, NAG-1 induction and its effect on the apoptosis in human oral cavity cancer cells (SCC 1483) have not been determined.

Materials and Method:NAG-1 expression by various NSAIDs in SCC 1483 cells was investigated by Western blot analysis. The induction of apoptosis by NSAID and the relationship between NAG-1 expression and apoptosis were determined by Western blot assay and flow cytometry. Drosophila cells stably expressing NAG-1 were constructed and NAG-1 conditioned medium (NCM) were made. Apoptosis was examined with flow cytometry on SCC 1483 cells treated with NCM.

Results:Diclofenac was the most potent inducer of NAG-1. Diclofenac inhibited the proliferation of SCC 1483 cells and this inhibition was proved as apoptosis. Diclofenac induced the expression of NAG-1 and also induced apoptosis in time and dose dependent manner. In the concentrated NCM, the expression of NAG-1 was intense and apoptosis was induced by addition of 5 μl of NCM.

Conclusion:Based on these data, we could assure that NSAIDs induced NAG-1 in oral cavity cancer cells and NAG-1 induced apoptosis. Therefore, we suggest that it is possible to use NSAIDs as a chemopreventive agent in oral cavity cancer. Further studies on the mechanism of NAG-1 and clinical use will be needed.
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1. College of Medicine (의과대학) > Dept. of Otorhinolaryngology (이비인후과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Kyung Su(김경수) ORCID logo https://orcid.org/0000-0003-1460-0640
Yoon, Joo Heon(윤주헌)
Lee, Jeung Gweon(이정권)
Lee, Joo Hwan(이주환)
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