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사람 구강암종 세포주에서 Non-Steroidal Anti-Inflammatory Drug-Activated Gene(NAG-1)의 유도 및 세포고사 작용
DC Field | Value | Language |
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dc.contributor.author | 김경수 | - |
dc.contributor.author | 윤주헌 | - |
dc.contributor.author | 이정권 | - |
dc.contributor.author | 이주환 | - |
dc.date.accessioned | 2015-07-14T17:00:12Z | - |
dc.date.available | 2015-07-14T17:00:12Z | - |
dc.date.issued | 2004 | - |
dc.identifier.issn | 1225-035X | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/112012 | - |
dc.description.abstract | Background and Objectives:Nonsteroidal anti-inflammatory drug (NSAID)-activated gene (NAG-1), which is induced by NSAIDs, has proapoptotic and antitumorigenic effects in colorectal cancer cells. However, NAG-1 induction and its effect on the apoptosis in human oral cavity cancer cells (SCC 1483) have not been determined. Materials and Method:NAG-1 expression by various NSAIDs in SCC 1483 cells was investigated by Western blot analysis. The induction of apoptosis by NSAID and the relationship between NAG-1 expression and apoptosis were determined by Western blot assay and flow cytometry. Drosophila cells stably expressing NAG-1 were constructed and NAG-1 conditioned medium (NCM) were made. Apoptosis was examined with flow cytometry on SCC 1483 cells treated with NCM. Results:Diclofenac was the most potent inducer of NAG-1. Diclofenac inhibited the proliferation of SCC 1483 cells and this inhibition was proved as apoptosis. Diclofenac induced the expression of NAG-1 and also induced apoptosis in time and dose dependent manner. In the concentrated NCM, the expression of NAG-1 was intense and apoptosis was induced by addition of 5 μl of NCM. Conclusion:Based on these data, we could assure that NSAIDs induced NAG-1 in oral cavity cancer cells and NAG-1 induced apoptosis. Therefore, we suggest that it is possible to use NSAIDs as a chemopreventive agent in oral cavity cancer. Further studies on the mechanism of NAG-1 and clinical use will be needed. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 1030~1037 | - |
dc.relation.isPartOf | Korean Journal of Otolaryngology (대한이비인후과학회지) | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | 사람 구강암종 세포주에서 Non-Steroidal Anti-Inflammatory Drug-Activated Gene(NAG-1)의 유도 및 세포고사 작용 | - |
dc.title.alternative | Induction of Non-Steroidal Anti-Inflammatory Drug-Activated Gene(NAG-1) in Human Oral Cavity Cancer Cells and its Effect on Apoptosis | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Otorhinolaryngology (이비인후과학) | - |
dc.contributor.googleauthor | 이주환 | - |
dc.contributor.googleauthor | 윤주헌 | - |
dc.contributor.googleauthor | 김경수 | - |
dc.contributor.googleauthor | 이윤재 | - |
dc.contributor.googleauthor | 장정현 | - |
dc.contributor.googleauthor | 이정권 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.relation.journalcode | J02088 | - |
dc.subject.keyword | Non-steroidal anti-inflammatory agents | - |
dc.subject.keyword | Oral cancers | - |
dc.subject.keyword | Apoptosis | - |
dc.contributor.alternativeName | Kim, Kyung Su | - |
dc.contributor.alternativeName | Yoon, Joo Heon | - |
dc.contributor.alternativeName | Lee, Jeung Gweon | - |
dc.contributor.alternativeName | Lee, Joo Hwan | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 47 | - |
dc.citation.number | 10 | - |
dc.citation.startPage | 1030 | - |
dc.citation.endPage | 1037 | - |
dc.identifier.bibliographicCitation | Korean Journal of Otolaryngology (대한이비인후과학회지), Vol.47(10) : 1030-1037, 2004 | - |
dc.identifier.rimsid | 52886 | - |
dc.type.rims | ART | - |
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