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마취유도제인 Thiopental이 쥐 심실근 세포의 Transient Outward Current (Ito) 및 Inwardly Rectifying K+ Current (Ik1)에 미치는 영향

Other Titles
 Effect of Thiopental on Transient Outward Current (Ito) and Inwardly Rectifying K+ Current (Ik1) in Rat Ventricular Myocytes 
 김명희  ;  윤경봉  ;  박윤곤  ;  이원재  ;  이경민 
 KOREAN JOURNAL OF ANESTHESIOLOGY, Vol.47(5) : 732-740, 2004 
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Issue Date
thiopental ; rat ; ventricular myocytes ; Ito ; Ik1 ; action potential
BACKGROUND: Patients with the long QT syndrome, either congenital or acquired, have an increased development of serious ventricular arrhythmia, Torsade de Pointes. Thiopental (5 mg/kg) has been reported to prolong the QTc interval in patients undergoing surgery with normal repolarization. Recent studies have indicated that the clinical concentration of thiopental prolonged the action potential duration (APD), which was attributed to inhibition of the delayed rectifier (Ik) and/or the inward rectifier (Ik1) at various animal myocardial preparations. The rat ventricular cells were used to study the contribution of transient outward current (Ito) and Ik1 because they possess a variety of K+channel subtypes including Ito and Ik1 with little or no Ik, similar to those of human ventricular myocytes. The effect on Ca2+ current (ICa,L), which can alter the K+ conductance, was also observed. METHODS: With approval of the animal research committee in Yonsei University Medical College, isolated ventricular cells were obtained from enzymatically treated rat hearts. The ICa,L was elicited from a holding potential of -40 mV to + 60 mV under the modified Tyrode solution. Ik1 was obtained from a holding potential of -40 mV before their membrane potential was changed from -130 to + 50 mV. Ito was recorded during depolarizing steps from -80 mV followed by inactivation of Na+current by short pulses to -40 mV and then depolarized with 10 mV increments to test potentials up to + 60 mV. ICa,L was blocked by adding 0.5 mM CdCl2 during measurement of Ito. Normal action potential was measured using conventional microelectrode technique. RESULTS: At membrane potential of +60 mV, 50microM thiopental caused modest depression of Ito to 82 ± 1% of control. From the dose-response curve from 1 to 1000microM, the IC50 of thiopental was 163microM. While 50microM thiopental caused modest depression of Ik1 of 87 ± 2% of control at a test potential of -130 mV, no depression was observed from -110 mV to + 50 mV. ICa,L was significantly reduced to 57 ± 5% of control. The APD90 was prolonged by 76% following application of 50microM thiopental. CONCLUSIONS: Prolongation of APD induced by thiopental was associated with reduction of Ito. Considering the high current density of Ito in rat ventricular myocytes, inhibition of Ito seems to play a major role in thiopental-induced APD prolongation.
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1. College of Medicine (의과대학) > Dept. of Anesthesiology and Pain Medicine (마취통증의학교실) > 1. Journal Papers
Yonsei Authors
Park, Wyun Kon(박윤곤)
Yoon, Kyoung Bong(윤경봉) ORCID logo https://orcid.org/0000-0002-4167-1375
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