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Chondrogenic differentiation of human mesenchymal stem cells using a thermosensitive poly(N-isopropylacrylamide) and water-soluble chitosan copolymer

Authors
 Jae Ho Cho  ;  Su-Hyang Kim  ;  Jin Woo Lee  ;  Ji Yeun Noh  ;  Sang Won Han  ;  Woo Ick Yang  ;  Myung Chul Jung  ;  Ki Dong Park 
Citation
 BIOMATERIALS, Vol.25(26) : 5743-5751, 2004 
Journal Title
 BIOMATERIALS 
ISSN
 0142-9612 
Issue Date
2004
MeSH
Absorbable Implants ; Acrylic Resins/chemistry* ; Biocompatible Materials/chemistry ; Cell Culture Techniques/methods ; Cell Differentiation/physiology ; Cell Proliferation ; Cell Survival/physiology ; Cells, Cultured ; Chitosan/chemistry* ; Chondrocytes/cytology* ; Chondrocytes/physiology* ; Humans ; Materials Testing ; Mesenchymal Stem Cells/cytology* ; Mesenchymal Stem Cells/physiology* ; Polymers/chemical synthesis ; Solubility ; Temperature ; Tissue Engineering/methods* ; Water/chemistry
Keywords
Thermally responsive material ; Chitosan ; Mesenchymal stem cell ; Sol–gel technique ; Poly(N-isopropylacrylamide)
Abstract
Poly(N-isopropylacrylamide) (PNIPAAm) is known to be thermally responsive material and has a lower critical solution temperature (LCST, 32 degrees C) at which a macromolecular transition from a hydrophilic to a hydrophobic structure occurs. Chitosan is a useful natural polymeric biomaterial due to its biocompatibility and biodegradable properties. It has good characteristics for cell attachment, proliferation and viability. The aim of this study was to assess the ability to differentiate from mesenchymal stem cells (MSCs) to chondrocytes and mass formation using a newly developed injectable material, a thermosensitive (water-soluble chitosan-g-PNIPAAm) gel, and evaluate cartilage formation in vivo after injecting a cell-thermosensitive gel complex. The MSCs were cultured in the chitosan-PNIPAAm in vitro. Fluorescence-activated cell sort analysis, viability test, collagen type I, II, X formation and the aggrecan levels were examined. These cultured cells can be easily recovered from a copolymer gel by simply lowering the temperature. An animal study was performed to assess cartilage formation in the submucosal layer of the bladder of rabbits. The cartilage formation could be detected. This can be used to treat vesicoureteral reflux or reflux esophagitis by the effective mass effect. This is a simple method (sol-gel technique in LCST), and good cartilage formation occurs in the bladder tissue.
Full Text
http://www.sciencedirect.com/science/article/pii/S014296120400064X
DOI
10.1016/j.biomaterials.2004.01.051
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Urology (비뇨의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Orthopedic Surgery (정형외과학교실) > 1. Journal Papers
Yonsei Authors
Yang, Woo Ick(양우익) ORCID logo https://orcid.org/0000-0002-6084-5019
Lee, Jin Woo(이진우) ORCID logo https://orcid.org/0000-0002-0293-9017
Han, Sang Won(한상원) ORCID logo https://orcid.org/0000-0003-0941-1300
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/111620
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