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Chondrogenic differentiation of human mesenchymal stem cells using a thermosensitive poly(N-isopropylacrylamide) and water-soluble chitosan copolymer

DC Field Value Language
dc.contributor.author양우익-
dc.contributor.author이진우-
dc.contributor.author한상원-
dc.date.accessioned2015-07-14T16:48:12Z-
dc.date.available2015-07-14T16:48:12Z-
dc.date.issued2004-
dc.identifier.issn0142-9612-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/111620-
dc.description.abstractPoly(N-isopropylacrylamide) (PNIPAAm) is known to be thermally responsive material and has a lower critical solution temperature (LCST, 32 degrees C) at which a macromolecular transition from a hydrophilic to a hydrophobic structure occurs. Chitosan is a useful natural polymeric biomaterial due to its biocompatibility and biodegradable properties. It has good characteristics for cell attachment, proliferation and viability. The aim of this study was to assess the ability to differentiate from mesenchymal stem cells (MSCs) to chondrocytes and mass formation using a newly developed injectable material, a thermosensitive (water-soluble chitosan-g-PNIPAAm) gel, and evaluate cartilage formation in vivo after injecting a cell-thermosensitive gel complex. The MSCs were cultured in the chitosan-PNIPAAm in vitro. Fluorescence-activated cell sort analysis, viability test, collagen type I, II, X formation and the aggrecan levels were examined. These cultured cells can be easily recovered from a copolymer gel by simply lowering the temperature. An animal study was performed to assess cartilage formation in the submucosal layer of the bladder of rabbits. The cartilage formation could be detected. This can be used to treat vesicoureteral reflux or reflux esophagitis by the effective mass effect. This is a simple method (sol-gel technique in LCST), and good cartilage formation occurs in the bladder tissue.-
dc.description.statementOfResponsibilityopen-
dc.format.extent5743~5751-
dc.relation.isPartOfBIOMATERIALS-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAbsorbable Implants-
dc.subject.MESHAcrylic Resins/chemistry*-
dc.subject.MESHBiocompatible Materials/chemistry-
dc.subject.MESHCell Culture Techniques/methods-
dc.subject.MESHCell Differentiation/physiology-
dc.subject.MESHCell Proliferation-
dc.subject.MESHCell Survival/physiology-
dc.subject.MESHCells, Cultured-
dc.subject.MESHChitosan/chemistry*-
dc.subject.MESHChondrocytes/cytology*-
dc.subject.MESHChondrocytes/physiology*-
dc.subject.MESHHumans-
dc.subject.MESHMaterials Testing-
dc.subject.MESHMesenchymal Stem Cells/cytology*-
dc.subject.MESHMesenchymal Stem Cells/physiology*-
dc.subject.MESHPolymers/chemical synthesis-
dc.subject.MESHSolubility-
dc.subject.MESHTemperature-
dc.subject.MESHTissue Engineering/methods*-
dc.subject.MESHWater/chemistry-
dc.titleChondrogenic differentiation of human mesenchymal stem cells using a thermosensitive poly(N-isopropylacrylamide) and water-soluble chitosan copolymer-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Urology (비뇨기과학)-
dc.contributor.googleauthorJae Ho Cho-
dc.contributor.googleauthorSu-Hyang Kim-
dc.contributor.googleauthorJin Woo Lee-
dc.contributor.googleauthorJi Yeun Noh-
dc.contributor.googleauthorSang Won Han-
dc.contributor.googleauthorWoo Ick Yang-
dc.contributor.googleauthorMyung Chul Jung-
dc.contributor.googleauthorKi Dong Park-
dc.identifier.doi10.1016/j.biomaterials.2004.01.051-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.relation.journalcodeJ00312-
dc.identifier.eissn1878-5905-
dc.identifier.pmid15147820-
dc.identifier.urlhttp://www.sciencedirect.com/science/article/pii/S014296120400064X-
dc.subject.keywordThermally responsive material-
dc.subject.keywordChitosan-
dc.subject.keywordMesenchymal stem cell-
dc.subject.keywordSol–gel technique-
dc.subject.keywordPoly(N-isopropylacrylamide)-
dc.contributor.alternativeNameYang, Woo Ick-
dc.contributor.alternativeNameLee, Jin Woo-
dc.contributor.alternativeNameHan, Sang Won-
dc.rights.accessRightsnot free-
dc.citation.volume25-
dc.citation.number26-
dc.citation.startPage5743-
dc.citation.endPage5751-
dc.identifier.bibliographicCitationBIOMATERIALS, Vol.25(26) : 5743-5751, 2004-
dc.identifier.rimsid37369-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Orthopedic Surgery (정형외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Urology (비뇨의학교실) > 1. Journal Papers

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