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German cockroach extract activates protease-activated receptor 2 in human airway epithelial cells

Authors
 Jeong Hee Hong  ;  Syng-Ill Lee  ;  Dong Min Shin  ;  Myung Hyun Sohn  ;  Jeong Taeg Seo  ;  Tai-Soon Yong  ;  Kyu-Earn Kim 
Citation
 JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, Vol.113(2) : 315-319, 2004 
Journal Title
 JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY 
ISSN
 0091-6749 
Issue Date
2004
MeSH
Allergens/immunology ; Animals ; Bronchi/cytology ; Bronchi/immunology* ; Calcium Signaling*/drug effects ; Cell Line ; Cockroaches/enzymology ; Cockroaches/immunology* ; Endopeptidases/immunology* ; Endopeptidases/pharmacology ; Epithelial Cells/immunology* ; Humans ; Receptor, PAR-2/metabolism* ; Thapsigargin/metabolism
Keywords
German cockroach ; Ca2+ signaling ; protease-activated receptor ; allergen ; human airway epithelial cell
Abstract
BACKGROUND: The German cockroach has been reported to act as an allergen that might be associated with a protease reaction in asthma. However, the molecular identities of the antigens in German cockroach extract (GCE) with protease activity and the protease-activated receptors (PARs) that are activated by GCE in human airway epithelial cells have not been characterized. OBJECTIVE: We investigated the direct effect of GCE on Ca(2+) signaling in human airway epithelial cells and the type of PARs activated by GCE. METHODS: The Ca(2+)-sensitive dye Fura2 was used to determine intracellular Ca(2+) concentration ([Ca(2+)](i)) by means of spectrofluorometry. RESULTS: GCE induced a baseline type of [Ca(2+)](i) oscillations in a dose-dependent manner. The oscillations persisted for long periods of time in the absence of Ca(2+) entry across the plasma membrane, suggesting that the observed [Ca(2+)](i) increases were due to Ca(2+) release from intracellular stores. Accordingly, after depleting endoplasmic reticulum Ca(2+) with thapsigargin, an endoplasmic reticulum Ca(2+) ATPase inhibitor, the GCE-mediated [Ca(2+)](i) signals were abolished. Whereas desensitization of PAR-1, PAR-3, and PAR-4 had no effect on GCE-mediated Ca(2+) mobilization, no GCE-mediated [Ca(2+)](i) increase was observed after desensitization of PAR-2. CONCLUSIONS: These results indicate that GCE has a direct effect on human airway epithelial cells, in particular generating [Ca(2+)](i) oscillations through Ca(2+) release from thapsigargin-sensitive Ca(2+) stores through activation of PAR-2.
Full Text
http://www.sciencedirect.com/science/article/pii/S0091674903026885
DOI
10.1016/j.jaci.2003.11.026
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Environmental Medical Biology (환경의생물학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pediatrics (소아청소년과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Kyu Earn(김규언)
Seo, Jeong Taeg(서정택) ORCID logo https://orcid.org/0000-0003-2697-0251
Sohn, Myung Hyun(손명현) ORCID logo https://orcid.org/0000-0002-2478-487X
Shin, Dong Min(신동민) ORCID logo https://orcid.org/0000-0001-6042-0435
Yong, Tai Soon(용태순) ORCID logo https://orcid.org/0000-0002-3445-0769
Lee, Syng Ill(이승일)
Hong, Jeong Hee(홍정희)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/111615
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