0 419

Cited 34 times in

Activation of NF-κB is involved in 6-hydroxydopamine—but not MPP+-induced dopaminergic neuronal cell death: its potential role as a survival determinant

Authors
 Seong H. Park  ;  Won-Seok Choi  ;  Young J. Oh  ;  Young Soo Ahn  ;  So-Young Yoon 
Citation
 BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Vol.322(3) : 727-733, 2004 
Journal Title
 BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 
ISSN
 0006-291X 
Issue Date
2004
MeSH
1-Methyl-4-phenylpyridinium/toxicity* ; Animals ; Cell Line ; Cell Survival/drug effects ; Dopamine/physiology* ; Kinetics ; NF-kappa B/drug effects ; NF-kappa B/metabolism* ; Neuroglia/cytology ; Neuroglia/drug effects ; Neuroglia/physiology ; Neurons/cytology* ; Neurons/drug effects ; Neurons/physiology ; Oxidopamine/pharmacology* ; Protein Transport ; Pyrrolidines/pharmacology ; Thiocarbamates/pharmacology
Keywords
NF-κB ; 6-Hydroxydopamine ; MPP+ ; PDTC ; MN9D cells
Abstract
The nuclear factor-kappaB (NF-κB) family plays an important role in the control of the apoptotic response. Its activation has been demonstrated in both neurons and glial cells in many neurological disorders. In the present study, we specifically examined whether and to what extent NF-κB activation is involved in culture models of Parkinson’s disease following exposure of MN9D dopaminergic neuronal cells to 6-hydroxydopamine (6-OHDA) and 1-methyl-4-phenyl-4-phenylpyridinium ion (MPP+). Both analysis by immunocytochemistry and of immunoblots revealed that NF-κB–p65 was translocated into the nuclei following 6-OHDA but not MPP+-treatment. A time-dependent activation of NF-κB induced by 6-OHDA but not MPP+ was also demonstrated by an electrophoretic mobility shift assay. A competition assay indicated that not only NF-κB–p65 but also –p50 is involved in 6-OHDA-induced NF-κB activity. Co-treatment with an antioxidant, N-acetyl-l-cysteine, blocked 6-OHDA-induced activation of NF-κB signaling. In the presence of an NF-κB inhibitor, pyrrolidine dithiocarbamate (PDTC), 6-OHDA-induced cell death was accelerated while PDTC did not affect MPP+-induced cell death. Our data may point to a drug-specific activation of NF-κB as a survival determinant for dopaminergic neurons.
Full Text
http://www.sciencedirect.com/science/article/pii/S0006291X0401695X
DOI
10.1016/j.bbrc.2004.07.193
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pharmacology (약리학교실) > 1. Journal Papers
Yonsei Authors
Ahn, Young Soo(안영수)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/111552
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse

Links