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Relationship between Epstein-Barr virus-encoded RNA expression, apoptosis and lymphocytic infiltration in gastric carcinoma with lymphoid-rich stroma

 Cho M.-Y.  ;  Kim T.H.  ;  Park K.H.  ;  Jung W.H.  ;  Yi S.Y. 
 MEDICAL PRINCIPLES AND PRACTICE, Vol.13(6) : 353-360, 2004 
Journal Title
Issue Date
Adenocarcinoma/pathology ; Adenocarcinoma/virology* ; Apoptosis* ; Case-Control Studies ; Epstein-Barr Virus Infections/pathology* ; Female ; Humans ; Immunohistochemistry ; In Situ Hybridization ; In Situ Nick-End Labeling ; Korea/epidemiology ; Lymphocytes, Tumor-Infiltrating/metabolism ; Lymphocytes, Tumor-Infiltrating/pathology* ; Male ; Middle Aged ; Proto-Oncogene Proteins c-bcl-2/metabolism ; RNA, Viral/analysis* ; Staining and Labeling ; Stomach Neoplasms/mortality ; Stomach Neoplasms/pathology ; Stomach Neoplasms/virology*
Carcinoma ; Stomach ; Gastric carcinoma with lymphoid-rich stroma ; Epstein-Barr virus ; Apoptosis ; bcl-2 ; bax
OBJECTIVE: To investigate the association of apoptosis with apoptotic proteins and Epstein-Barr virus (EBV) expression in gastric carcinoma with lymphoid-rich stroma (GCLRS) in order to clarify the relationship between lymphocytic infiltration, EBV expression and apoptosis in gastric carcinoma. SUBJECTS and METHODS: We performed immunohistochemical stains for bcl-2, bax and in situ hybridization for EBV-encoded RNA and the terminal deoxynucleotidyl transferase-mediated dUTP-digoxigenin nick end labeling technique using the 24 cases of GCLRS and 23 cases of gastric carcinoma with lymphoid-poor stroma (GCLPS) out of 1,635 cases of gastric carcinoma. RESULTS: The prevalence of GCLRS was 1.47% (24/1,635). The prevalence of EBV involvement in GCLRS (79%, 19/24) was significantly higher than in GCLPS (8.7%, 2/23). Apoptosis was revealed in all cases and there was no statistical difference between GCLRS and GCLPS. Immunohistochemical expression for bcl-2 was revealed only in the cytoplasm of lymphocytes in all cases, but bax showed distinct expression in the cytoplasm of neoplastic cells in all cases. There was a significant correlation between bax expression and the apoptotic index (p <0.05). Lymphocytic infiltration was significantly correlated with the expression of EBV (p <0.05) but not with survival rate and apoptosis. Most of the GCLRS cases were rated as low clinical stage (stage IA or B) and seemed to have a good prognosis. CONCLUSIONS: These results support the hypothesis that EBV infection plays an important role in the tumorigenesis of GCLRS, which has a better prognosis than usual gastric adenocarcinoma although there was no significant difference in the apoptotic index between GCLRS and GCLPS.
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1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
Yonsei Authors
Jung, Woo Hee(정우희)
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