Cited 10 times in
Allergen-specific immunosuppression by ovalbumin fused with diphtheria toxin in mice sensitized with albumins of different origin
DC Field | Value | Language |
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dc.contributor.author | 김주영 | - |
dc.contributor.author | 노재열 | - |
dc.contributor.author | 유정은 | - |
dc.contributor.author | 이봉기 | - |
dc.contributor.author | 장윤수 | - |
dc.contributor.author | 홍천수 | - |
dc.date.accessioned | 2015-07-14T16:40:22Z | - |
dc.date.available | 2015-07-14T16:40:22Z | - |
dc.date.issued | 2004 | - |
dc.identifier.issn | 0954-7894 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/111358 | - |
dc.description.abstract | BACKGROUND: We previously reported that ovalbumin-diphtheria toxin (OVA-DT) fusion protein eliminates mast cells bearing OVA-specific IgE and protects OVA-sensitized mice from fatal anaphylaxis induced by OVA challenge. OBJECTIVE: To prove the specificity of therapeutic effect of OVA-DT to allergy induced by OVA only and not by other allergens such as human serum albumin (HSA), and to examine the cytotoxic effect of OVA-DT on B cells bearing OVA-specific IgE. METHODS: Mice were sensitized with two different antigens, OVA and HSA, and then treated with OVA-DT. The therapeutic effect of OVA-DT on the allergy response to each of allergen was evaluated by anaphylactic test. The effect of OVA-DT on the production of allergen-specific Ig isotypes of the sensitized mice and the cytotoxic effect of OVA-DT on B cells expressing OVA-specific IgE were examined. RESULTS: OVA-DT suppressed only OVA-induced allergy but not HSA-induced allergy in mice sensitized with a mixture of OVA and HSA. The suppression was prolonged even to the mice boosted with the same allergen 14 days after last treatment of OVA-DT. In addition, when the sensitized mice were boosted with the same allergens 14 days after last treatment of OVA-DT, the mice showed to increase the production of OVA-specific IgG2a/IgG3 and decreased that of OVA-specific IgE. OVA-DT targeted B cells bearing OVA-specific IgE, and killed them by DT-mediated cytotoxicity. CONCLUSION: The therapeutic effect of OVA-DT was specific to OVA-induced allergy and the suppression of OVA-induced allergy was continuously shown in the mice boosted with the same allergens. This is considered to be caused by the increase of OVA-specific IgG2a and IgG3, and because of the decrease of OVA-specific IgE by killing of B cells bearing OVA-specific IgE. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 1642~1648 | - |
dc.relation.isPartOf | CLINICAL AND EXPERIMENTAL ALLERGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Allergen-specific immunosuppression by ovalbumin fused with diphtheria toxin in mice sensitized with albumins of different origin | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Pharmacology (약리학) | - |
dc.contributor.googleauthor | B. K. Lee | - |
dc.contributor.googleauthor | J. E. Yoo | - |
dc.contributor.googleauthor | J. Y. Ro | - |
dc.contributor.googleauthor | C. S. Hong | - |
dc.contributor.googleauthor | J. Y. Kim | - |
dc.contributor.googleauthor | Y. S. Jang | - |
dc.identifier.doi | 10.1111/j.1365-2222.2004.02077.x | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.relation.journalcode | J00548 | - |
dc.identifier.eissn | 1365-2222 | - |
dc.identifier.url | http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2222.2004.02077.x/abstract | - |
dc.subject.keyword | allergen–toxin fusion protein | - |
dc.subject.keyword | diphtheria toxin | - |
dc.subject.keyword | immunotherapy | - |
dc.subject.keyword | ovalbumin | - |
dc.contributor.alternativeName | Kim, Joo Young | - |
dc.contributor.alternativeName | Ro, Jai Youl | - |
dc.contributor.alternativeName | Yoo, Jeong Eun | - |
dc.contributor.alternativeName | Lee, Bong Ki | - |
dc.contributor.alternativeName | Jang, Y. S. | - |
dc.contributor.alternativeName | Hong, Chein Soo | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 34 | - |
dc.citation.number | 10 | - |
dc.citation.startPage | 1642 | - |
dc.citation.endPage | 1648 | - |
dc.identifier.bibliographicCitation | CLINICAL AND EXPERIMENTAL ALLERGY, Vol.34(10) : 1642-1648, 2004 | - |
dc.identifier.rimsid | 35999 | - |
dc.type.rims | ART | - |
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