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Expression of psoriasis-associated fatty acid-binding protein in senescent human dermal microvascular endothelial cells

 Moon Kyung Ha  ;  Kee Yang Chung  ;  Kwang Hoon Lee  ;  Yoon Kee Park  ;  Dongsik Bang  ;  Ju Hee Lee 
 EXPERIMENTAL DERMATOLOGY, Vol.13(9) : 543-550, 2004 
Journal Title
Issue Date
Antibody Specificity ; Biomarkers ; Carrier Proteins/immunology ; Carrier Proteins/metabolism* ; Cell Division ; Cells, Cultured ; Cross Reactions ; Dermis/blood supply ; Dermis/metabolism ; Electrophoresis, Gel, Two-Dimensional ; Endothelial Cells/metabolism* ; Endothelial Cells/ultrastructure ; Fatty Acid-Binding Proteins ; Flow Cytometry ; Humans ; Immunohistochemistry ; Microscopy, Confocal ; Neoplasm Proteins/immunology ; Neoplasm Proteins/metabolism* ; Skin Aging/physiology* ; beta-Galactosidase
aging ; cellular senescence ; endothelial cells ; fatty acid-binding protein ; proteomics
Aging is associated with the progressive pathophysiologic modification of endothelial cells. In vitro endothelial cell senescence is accompanied by proliferative activity failure and by perturbations in gene and protein expressions. Moreover, this cellular senescence in culture has been proposed to reflect processes that occur in aging organisms. In order to observe the changing patterns of protein expression in senescent human dermal microvascular endothelial cells (HDMECs), proteins obtained from both early- and late-passaged HDMECs were separated by two-dimensional electrophoresis, visualized by silver staining, and quantified by image processing. Proteins of interest were extracted by in-gel digestion with trypsin and quantified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS), by searching the National Center for Biotechnology Information protein-sequence database. More than 2000 spots were detected by 2D electrophoresis within a linear pH range of 3–10. Twenty-two major differentially expressed spots were observed in serially passaged HDMECs and identified with high confidence by MALDI-TOF-MS. One of these spots was found to be a 14–15 kDa psoriasis-associated fatty acid-binding protein (PA-FABP) with high affinity for long-chain fatty acids. The expression of PA-FABP was confirmed to be elevated in senescent HDMECs (passage 20) by fluorescence-activated cell sorting (FACS), confocal laser microscopy, and by immunohistochemistry in aged human skin tissue. Our results suggest that the overexpression of FABP in cultured senescent HDMECs is closely related to skin aging.
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1. College of Medicine (의과대학) > Dept. of Dermatology (피부과학교실) > 1. Journal Papers
Yonsei Authors
Park, Yoon Kee(박윤기)
Lee, Kwang Hoon(이광훈)
Lee, Ju Hee(이주희) ORCID logo https://orcid.org/0000-0002-1739-5956
Chung, Kee Yang(정기양) ORCID logo https://orcid.org/0000-0003-3257-0297
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