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The myoepithelial cell differentiation of mucoepidermoid carcinoma in a collagen gel-based coculture model

Authors
 Jong In Yook  ;  Seoung Ae Lee  ;  Jin Kim  ;  In Ho Cha  ;  Jung Huh  ;  Yong Chan Chun 
Citation
 JOURNAL OF ORAL PATHOLOGY & MEDICINE, Vol.33(4) : 237-242, 2004 
Journal Title
 JOURNAL OF ORAL PATHOLOGY & MEDICINE 
ISSN
 0904-2512 
Issue Date
2004
MeSH
3T3 Cells ; Actins/analysis ; Adult ; Animals ; Carcinoma, Mucoepidermoid/pathology* ; Carcinoma, Mucoepidermoid/secondary ; Carcinoma, Squamous Cell/pathology ; Cell Differentiation ; Cell Line, Tumor ; Coculture Techniques ; Codon/genetics ; Collagen ; Culture Media ; Epithelial Cells/pathology ; Genes, p53/genetics ; Humans ; Keratins/analysis ; Lymphatic Metastasis/pathology ; Male ; Mice ; Mice, Inbred BALB C ; Mice, Nude ; Muscle, Smooth/pathology* ; Neoplasm Transplantation ; Point Mutation/genetics ; Tongue Neoplasms/pathology
Keywords
collagen gel‐based coculture ; microenvironment ; mucoepidermoid carcinoma ; mucous cells ; myoepithelial cells ; squamous cells ; transplantation
Abstract
BACKGROUND: Mucoepidermoid carcinomas (MECs) are the most common malignant tumor of the salivary glands; however, the histogenesis of MECs has been still controversial. This study was undertaken to investigate the histogenesis of MECs by the examination of their collagen gel-based coculture tissue and transplanted tumors. METHODS: Two cell lines from a primary and a metastatic MECs were established and characterized by the mutational analysis of the p53 gene and in vivo tumorigenicity in athymic nude mice. Collagen gel-based organotypic cocultures were performed, and the ultrastructural and immunohistochemical findings were examined. RESULTS: Two cell lines demonstrated p53 point mutation at the same codon. A metastatic cell line of MEC showed in vivo tumorigenicity. Transplanted tumors and the collagen gel-based culture tissues showed poorly differentiated squamous cell carcinomas devoid of mucous cell differentiation; however, they disclosed the differentiation of myoepithelial cells. CONCLUSIONS: MECs appear to be centered on the squamous cell differentiation, and the specific differentiation of myoepithelial or mucous cells seems to be modulated by the property of microenvironment.
Full Text
http://onlinelibrary.wiley.com/doi/10.1111/j.0904-2512.2004.00056.x/abstract
DOI
10.1111/j.0904-2512.2004.00056.x
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Oral Pathology (구강병리학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Oral and Maxillofacial Surgery (구강악안면외과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Jin(김진)
Yook, Jong In(육종인) ORCID logo https://orcid.org/0000-0002-7318-6112
Cha, In Ho(차인호) ORCID logo https://orcid.org/0000-0001-8259-2190
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/111344
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