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Hydrogenosomal activity of Trichomonas vaginalis cultivated under different iron conditions

Authors
 Yong-seok Kim  ;  Hyun-ouk Song  ;  Ik-hwa Choi  ;  Soon-jung Park  ;  Jae-sook Ryu 
Citation
 KOREAN JOURNAL OF PARASITOLOGY, Vol.44(4) : 373-378, 2006 
Journal Title
KOREAN JOURNAL OF PARASITOLOGY
ISSN
 0023-4001 
Issue Date
2006
MeSH
Animals ; Culture Media ; Ferredoxins/genetics ; Ferredoxins/metabolism ; Gene Expression Regulation* ; Gene Expression Regulation, Enzymologic ; Humans ; Hydrogen/metabolism* ; Hydrogenase/genetics ; Hydrogenase/metabolism ; Iron/metabolism* ; Malate Dehydrogenase/genetics ; Malate Dehydrogenase/metabolism ; Membrane Potentials ; Organelles/enzymology* ; Organelles/metabolism ; Organelles/physiology* ; Pyruvate Synthase/genetics ; Pyruvate Synthase/metabolism ; Reverse Transcriptase Polymerase Chain Reaction ; Trichomonas vaginalis/growth & development*
Keywords
Trichomonas vaginalis ; hydrogenosomal enzyme ; gene expression> ; hydrogenosomal membrane potential ; iron ; DiOC6 ; PFOR ; hydrogenase ; malic enzyme ; ferredoxin
Abstract
To evaluate whether iron concentration in TYM medium influence on hydrogenosomal enzyme gene expression and hydrogenosomal membrane potential of Trichomonas vaginalis, trophozoites were cultivated in iron-depleted, normal and iron-supplemented TYM media. The mRNA of hydrogenosomal enzymes, such as pyruvate ferredoxin oxidoreductase (PFOR), hydrogenase, ferredoxin and malic enzyme, was increased with iron concentrations in T. vaginalis culture media, measured by RT-PCR. Hydrogenosomal membrane potentials measured with DiOC6 also showed similar tendency, e.g. T. vaginalis cultivated in iron-depleted and iron-supplemented media for 3 days showed a significantly reduced and enhanced hydrogenosomal membrane potential compared with that of normal TYM media, respectively. Therefore, it is suggested that iron may regulate hydrogenosomal activity through hydrogenosomal enzyme expression and hydrogenosomal membrane potential.
Files in This Item:
T200601647.pdf Download
DOI
10.3347/kjp.2006.44.4.373
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Tropica Medicine (열대의학교실) > 1. Journal Papers
Yonsei Authors
Park, Soon Jung(박순정) ORCID logo https://orcid.org/0000-0002-0423-1944
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/110657
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