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Streptococcus mutans의 선택성 향상을 위한 방법 고찰: 원심분리법과 선택 배지의 활용

Other Titles
 Improvement of selectivity for Streptococcus mutans by centrifugal procedure and selective media 
Authors
 김민영  ;  권호근  ;  김백일 
Citation
 Journal of Korean Academy of Oral Health (대한구강보건학회지), Vol.30(3) : 261-270, 2006 
Journal Title
Journal of Korean Academy of Oral Health(대한구강보건학회지)
ISSN
 1225-388x 
Issue Date
2006
Keywords
centrifugal force ; MSB ; MS-MUT ; slective culture ; Streptococcus mutans
Abstract
Objectives: The first purpose of this study was to evaluate differences in selective ability for S. mutans between existing MSB and MS-MUT which was recently developed by Hirasawa and Takada. The second purpose was to verify whether centrifugal forces were able to enhance selective ability in S. mutans selective media and to find out the optimized centrifugal force in vitro culture.
Methods: Salivary samples were collected from twenty five adults (Males: 13, Females: 12) and one milliliter of paraffin-stimulated saliva was collected for 5 minutes. MSB and MS-MUT media were used for S. mutans culture. All centrifugal conditions were in 4℃ and 15 minutes and centrifugal forces were 1000, 5000, 13000, and 20000 ×g. After the centrifugal process, we used with substratum layer for next procedure. The colony forming units (CFUs) of S. mutans and non-S. mutans in salivary samples were calculated by one experiened microbiologist. The t-test was used to compare CFUs between MSB and MS-MUT. The ANOVA test was used for evaluating of statistical significance in each centrifugal step.
Results: The CFUs of S. mutans in MSB media were similar to MS-MUT meida in non centrifugal salivary samples. However, CFUs of non-S. mutans in MSB were significantly lower than MS-MUT (P<0.05). The lower stratum of centrigugal salivary sample with 13000 ×g showed the maximum CFUs of S.mutans and minimum CFUs of non-S. mutans.
Conclusions: This results showed that existing MSB media was superior to new MS-MUT in selective culture of S. mutans. The centrifugal forces played an efficient role for S. mutans selective culture and the optimized centrifugal force was considered as 13,000 ×g.
Files in This Item:
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Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Preventive Dentistry and Public Oral Health (예방치과학교실) > 1. Journal Papers
Yonsei Authors
Kwon, Ho Keun(권호근)
Kim, Baek Il(김백일) ORCID logo https://orcid.org/0000-0001-8234-2327
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/110417
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