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Signaling for Integrin α5/β1 Expression in Helicobacter pylori–Infected Gastric Epithelial AGS Cells

Authors
 SOON OK CHO  ;  KYUNG HWAN KIM  ;  JOO-HEON YOON  ;  HYEYOUNG KIM 
Citation
 ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, Vol.1090 : 298-304, 2006 
Journal Title
 ANNALS OF THE NEW YORK ACADEMY OF SCIENCES 
ISSN
 0077-8923 
Issue Date
2006
MeSH
Epithelial Cells/metabolism ; Epithelial Cells/microbiology ; Gastric Mucosa/metabolism* ; Helicobacter Infections/metabolism* ; Helicobacter Infections/microbiology ; Helicobacter pylori/isolation & purification* ; Humans ; Integrin alpha5beta1/metabolism* ; Signal Transduction* ; Stomach/cytology ; Stomach/microbiology
Keywords
Helicobacter pylori ; integrin α5/β1 ; AGS cells
Abstract
Integrin expression in cancer tissues demonstrates its possible contribution to tumor progression, invasion, and metastasis. Helicobacter pylori (H. pylori) infection is related to gastric cancer and gastric inflammation. H. pylori induced upregulation in expression of integrin in gastric epithelia cells. Reactive oxygen species (ROS) are considered as an important regulator in the pathogenesis of H. pylori–induced gastric ulceration and carcinogenesis. Integrin expression may be regulated by oxidant-sensitive transcription factors, nuclear factor-κB (NF-κB) and activator protein-1 (AP-1). The present study aims to investigate whether H. pylori in a Korean isolate (HP99) induces the expression of integrin α5 and integrin β1, and whether H. pylori–induced expression of integrin α5 and integrin β1 are inhibited in the cells transfected with mutant genes for Ras (ras N-17), c-Jun (TAM-67), and IκBα(MAD-3) or treated with DPI, an inhibitor of NADPH oxidase. As a result, H. pylori induced the expression of integrin α5 and integrin β1 in gastric adenocarcinoma (AGS) cells time-dependently. Treatment of DPI or transfection with mutant genes for Ras (ras N-17), c-jun (TAM67), and IκBα(MAD3) inhibited H. pylori–induced expression of integrin α5 and integrin β1 in AGS cells. In conclusion, H. pylori activates Ras, NF-κB, and AP-1 and thus induces the expression of integrin α5 and integrin β1 in gastric epithelial cells. Inhibition of ROS production by DPI suppressed the expression of integrin α5 and integrin β1 in gastric epithelial cells. The results suggest the possible involvement of NADPH oxidase for ROS production in H. pylori–infected gastric epithelial cells.
Full Text
http://onlinelibrary.wiley.com/doi/10.1196/annals.1378.032/abstract
DOI
10.1196/annals.1378.032
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pharmacology (약리학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Otorhinolaryngology (이비인후과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Kyung Hwan(김경환)
Yoon, Joo Heon(윤주헌)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/110403
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