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A role of the amino-terminal (N) and carboxyl-terminal (C) interaction in binding of androgen receptor to chromatin

 Jiwen Li  ;  Junjiang Fu  ;  Charalambos Toumazou  ;  Ho-Geun Yoon  ;  Jiemin Wong 
 MOLECULAR ENDOCRINOLOGY , Vol.20(4) : 776-785, 2006 
Journal Title
Issue Date
Amino Acid Substitution ; Animals ; Base Sequence ; Binding Sites/genetics ; Cell Line ; Chromatin/genetics ; Chromatin/metabolism* ; DNA/genetics ; DNA/metabolism ; Female ; Humans ; In Vitro Techniques ; Multiprotein Complexes ; Oocytes/metabolism ; Receptors, Androgen/chemistry* ; Receptors, Androgen/genetics ; Receptors, Androgen/metabolism* ; Recombinant Proteins/chemistry ; Recombinant Proteins/genetics ; Recombinant Proteins/metabolism ; Sequence Deletion ; Transcription Factors/chemistry ; Transcription Factors/metabolism ; Transcriptional Activation ; Xenopus
The N-terminal domain of AR is known to engage a hormone-dependent interaction with its C-terminal ligand-binding domain, and this N/C interaction is known to modulate AR transcriptional activity. Using Xenopus oocytes as a model system to study transcriptional regulation in chromatin, we found that two previously reported N/C interaction-defective AR mutants, one with deletion of 23FQNLF27(ARΔF) and one with a Gly 21 to Glu mutation (ARG21E), were surprisingly inactive in activating transcription from various reporters assembled into chromatin. Further study using chromatin immunoprecipitation assay revealed that these mutants failed to bind both mouse mammary tumor virus-long terminal repeat and prostate-specific antigen enhancer assembled into chromatin. This defect is specific to chromatin because both mutants could bind to a consensus AR response element in vitro and activate transcription driven by mouse mammary tumor virus-long terminal repeat in transient transfection as effective as the wild-type AR. To further substantiate this novel finding, we established 293 cell lines that stably expressed either AR or ARΔF mutant in an inducible manner. Using these cell lines, we confirmed by using chromatin immunoprecipitation assay that AR but not ARΔF could bind to the endogenous prostate-specific antigen enhancer. Furthermore, we found that the ARΔF mutant interacts poorly with Brg1, the ATPase subunit of the chromatin-remodeling factor SWI/SNF. Taken together, our study reveals a novel role of AR N/C interaction in control of AR chromatin binding and suggests a working model that the proper N/C interaction is required for AR to recruit SWI/SNF complex, which in turn remodels chromatin to allow AR to bind to AR response elements in chromatin.
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1. College of Medicine (의과대학) > Dept. of Biochemistry and Molecular Biology (생화학-분자생물학교실) > 1. Journal Papers
Yonsei Authors
Yoon, Ho Geun(윤호근) ORCID logo https://orcid.org/0000-0003-2718-3372
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