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분해된 DNA검체 분석을 위한 9개의 Non-CODIS MiniSTR 유전좌의 유용성 평가

Other Titles
 Evaluation of Nine Non-CODIS MiniSTR Loci to aid Analysis of Degraded DNA 
 Ukhee Chung  ;  Hwan Young Lee  ;  Myung Jin Park  ;  Woo Ick Yang  ;  Sang-Ho Cho  ;  Kyoung-Jin Shin 
 Korean Journal of Legal Medicine (대한법의학회지), Vol.30(2) : 160-167, 2006 
Journal Title
Korean Journal of Legal Medicine(대한법의학회지)
Issue Date
miniSTR ; D1S1677 ; D2S441 ; D3S3053 ; D4S2364 ; D6S474 ; D10S1248 ; D14S1434 ; D20S482 ; D22S1045 ; Degraded DNAD20S482 ; D22S1045 ; Degraded DNA
For highly degraded DNA samples of forensic casework, new miniSTR PCR systems have been developed to supplement the current CODIS STRs. In the present study, we established the three miniplexes for nine miniSTRs (NC01 : D10S1248, D14S1434 and D22S1045; NC02 : D1S1677, D2S441 and D4S2364; and NC03 : D3S3053, D6S474 and D20S482) which had been previously suggested by Butler group (NIST, Gaitherburg, MD, USA). To evaluate the usefulness of the nine miniSTRs in analysis of degraded DNA, the sensitivity and efficacy of the three miniplexes were determined and then compared with those of the BigMini STR system which consists of six CODIS miniSTRs (TH01, CSF1PO, FGA, TPOX, D7S820, and D21S11). The three miniplexes gave better results in both the sensitivity test and efficiency test in comparison with BigMini. In the sensitivity test using serially diluted standard DNA, most loci in the three miniplexes showed reliable results for samples containing 50 pg of DNA and some even showed good sensitivity for samples containing 30 pg of DNA. Additionally, the three miniplexes generated useful profiles for both enzymatically degraded DNA and 50-year old skeletal remain samples. Among the nine miniSTRs, D4S2364, D3S3053, D14S1434, and D1S1677 produced the most successful DNA profiles for old skeletal remains. These results suggest that new miniSTRs could be useful supplements to the 13 CODIS STRs for forensic analysis of degraded DNA.
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1. College of Medicine (의과대학) > Dept. of Forensic Medicine (법의학과) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
Yonsei Authors
Shin, Kyoung Jin(신경진) ORCID logo https://orcid.org/0000-0002-1059-9665
Yang, Woo Ick(양우익) ORCID logo https://orcid.org/0000-0002-6084-5019
Cho, Sang Ho(조상호)
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