1 446

Cited 77 times in

Effect of estrogen on calcium-handling proteins, β-adrenergic receptors, and function in rat heart

Authors
 Sang Hui Chu  ;  Paul Goldspink  ;  Jill Kowalski  ;  Jenny Beck  ;  Dorie W. Schwertz 
Citation
 LIFE SCIENCES, Vol.79(13) : 1257-1267, 2006 
Journal Title
LIFE SCIENCES
ISSN
 0024-3205 
Issue Date
2006
MeSH
Animals ; Calcium/metabolism ; Calcium Channels, L-Type/metabolism ; Calcium-Binding Proteins/biosynthesis ; Calcium-Binding Proteins/metabolism* ; Estrogen Replacement Therapy ; Estrogens/pharmacology* ; Female ; Heart/drug effects* ; Heart Ventricles/drug effects ; Heart Ventricles/metabolism ; Homeostasis/drug effects ; Membrane Proteins/metabolism ; Muscle Proteins/biosynthesis ; Muscle Proteins/genetics ; Myocardial Contraction/drug effects ; Organ Size/drug effects ; Ovariectomy ; Papillary Muscles/drug effects ; RNA, Messenger/biosynthesis ; Rats ; Rats, Sprague-Dawley ; Receptors, Adrenergic, beta/drug effects* ; Sodium-Calcium Exchanger/metabolism
Keywords
Sex difference ; Gender ; Heart ; Ovariectomy ; Estrogen ; SERCA ; Phospholamban ; Contraction
Abstract
Regulation of cellular Ca2+ cycling is central to myocardial contractile function. Loss of Ca2+ regulation is associated with cardiac dysfunction and pathology. Estrogen has been shown to modify contractile function and to confer cardioprotection. Therefore, we investigated the effect of estrogen on expression of rat heart myocardial Ca2+-handling proteins and beta-adrenergic receptor (β1-AR) and examined functional correlates. Female rats were sham-operated (SHAM) or ovariectomized. Two weeks after ovariectomy rats were injected (i.p.) daily with estradiol benozoate (OVX + EB) or sesame oil (OVX) for 2 weeks. Protein abundance was measured by immunoblotting and mRNA was quantified by real-time RT-PCR. OVX significantly decreased estrogen and progesterone levels and EB replacement returned both estrogen and progesterone to physiological levels. OVX induced a 75% reduction of uterine weight and a gain in body weight. Replacement restored weights to SHAM level. OVX increased and estrogen-replacement normalized abundance of β1-AR and L-type Ca2+ channel (Cav1.2) protein. OVX decreased sodium–Ca2+ exchange protein (NCX) and estrogen restored protein abundance to SHAM levels. Sarcoplasmic reticular ATPase (SERCA), phospholamban (PLB), and ryanodine receptor (RyR) abundance was not altered by hormone status. Levels of mRNA encoding for β1-AR, Cav1.2, and NCX were not influenced by OVX or estrogen replacement. OVX had no effect on SERCA and PLB mRNA level but estrogen replacement elicited a significant increase compared to OVX and SHAM. Estrogen-dependent changes in Ca2+-handling proteins and β1-AR are theoretically consistent reduced myocellular Ca2+ load. However, hormone-dependent alterations in protein were not associated with changes in contractile function.
Full Text
http://www.sciencedirect.com/science/article/pii/S0024320506002967
DOI
10.1016/j.lfs.2006.03.037
Appears in Collections:
3. College of Nursing (간호대학) > Dept. of Nursing (간호학과) > 1. Journal Papers
Yonsei Authors
Chu, Sang Hui(추상희) ORCID logo https://orcid.org/0000-0001-6877-5599
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/110171
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse

Links