자일리톨이 함유된 curcuma xanthorrhiza 추출물이 streptococcus mutans 바이오필름에 미치는 효과

Abstract

Objectives: The purpose of this study was to evaluate the effect of Curcuma xanthorrhiza extract(CXE) containing xylitol on planktonic Streptococcus mutans (S. mutans) and S. mutans biofilms. Minimum inhibitory concentrations, the fast effectiveness antibacterial agents and antibacterial activity against S. mutans biofilms were examined for the evaluation. Methods: S. mutans were exposed to antibacterial agents at different concentrations(from 5 ppm to 5,000 ppm) for different time exposures(1, 2, 5, 10 minutes and 24 hours). Biofilm formation was quantified by a crystal violet assay. The amount of the crystal violet stain was measured with a tunable microplate reader at 655 nm. The activity of antibacterial agents were expressed as the optical density of biofilm. Results: MICs of CXE and CXE containing xylitol were 5 ppm, 10 ppm on planktonic S. mutans. However, xylitol could not find any MICs in concentration of this experiments. As a result of the fast effectiveness of antibacterial agents, CXE and CXE containing xylitol were able to completely inhibit growth of bacteria when they were exposed to these antibacterial agents for 1 minute in concentrations of 25 ppm, 50 ppm on planktonic S. mutans. However, xylitol couldn't inhibit growth of S. mutans. According to antibacterial activity of antibacterial agents against S. mutans biofilms, CXE and CXE containing xylitol inhibited S. mutans biofilm formation in concentration of 50 ppm. However, xylitol also couldn't inhibit growth of S. mutans biofilm formation. Conclusions: As the result of this study, CXE containing xylitol with a highly selective antibacterial activity appears to be an attractive candidate.