GnRH (Gonadotropin-Releasing Hormone)에 의한 자궁내막암 유래 세포주의 세포 증식 억제 기전에 있어서 Integrin, FAK (FocalAdhesion Kinase) 및 ERK (Extracellular Signal Regulated Kinase)의 역할

Abstract

Objective: To investigate new signal transduction cascade through integrin, FAK and ERK in the suppressed cell proliferation by GnRH-I and -II.

Method: Human endometrial cancer cells (HEC1A) were cultured under the following condition: DMEM/F12 (10% FBS). GnRH-I and -II were treated time (0, 5, 10, 15, 20, 30 min; 100 nM) and dose (10 nM or 100 nM; 20 min) dependent manner according to experimental purposes. Cell proliferation was measured using [3H] thymidine incorporation assay. Immunoblotting was utilized to detect proteins.

Results: GnRH-I and -II inhibited proliferation of HEC1A cells and induced expression of integrin β3. Phosphorylation of FAK and ERK were induced by GnRH-I and -II.

Conclusion: GnRH inhibited cell proliferation via the expression of integrin and FAK, ERK phosphorylation.