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Collagen-based co-culture for invasive study on cancer cells-fibroblasts interaction

Authors
 Zhong Min Che  ;  Tae Heob Jung  ;  Jung Hee Choi  ;  Do Jun Yoon  ;  Hyun Jeong Jeong  ;  Eun Ju Lee  ;  Jin Kim 
Citation
 BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Vol.346(1) : 268-275, 2006 
Journal Title
 BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 
ISSN
 0006-291X 
Issue Date
2006
MeSH
Cathepsin D/biosynthesis ; Cell Communication ; Cell Line, Tumor ; Coculture Techniques/methods* ; Collagen/pharmacology* ; Fibroblasts/cytology* ; Humans ; Matrix Metalloproteinase 2/biosynthesis ; Matrix Metalloproteinase 9/biosynthesis ; Neoplasm Invasiveness/physiopathology* ; Stromal Cells/cytology*
Keywords
Cancer cells-fibroblasts interaction ; Direct contact ; Indirect contact ; Collagen gel-based co-culture ; Matrix metalloproteinase ; Cathepsin D
Abstract
The roles of tumor stroma in carcinogenesis are still unclear. This study was aimed at designing an in vitro model for investigating the effects of stromal fibroblasts in the invasive growth of squamous cell carcinoma. Using two cancer cell lines, we performed three-dimensional co-culture with dermal equivalents to evaluate the effects of fibroblasts in cancer invasion. In vitro models for cellular interaction study were designed as follows: a collagen gel-based direct co-culture model (C-Dr) and a collagen gel-based indirect co-culture model (C-In). The invasive growth was found only in the dermal equivalents with fibroblasts. MMP-2 activity could be induced by direct contact between cancer cells and stromal fibroblasts. Cathepsin D was also highly expressed when co-cultured with cancer cells and fibroblasts. The present study demonstrated that the presence of fibroblasts is essential in cancer invasion and that collagen gel-based co-culture models might be useful for invasive study.
Full Text
http://www.sciencedirect.com/science/article/pii/S0006291X06011727
DOI
10.1016/j.bbrc.2006.05.111
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Oral Pathology (구강병리학교실) > 1. Journal Papers
5. Research Institutes (연구소) > Oral Cancer Research Institute (구강종양연구소) > 1. Journal Papers
Yonsei Authors
Kim, Jin(김진)
Lee, Eun Ju(이은주)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/109701
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