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Diaphragmatic repair through fetal tissue engineering: a comparison between mesenchymal amniocyte– and myoblast-based constructs

Authors
 Shaun M. Kunisaki  ;  Julie R. Fuchs  ;  Amir Kaviani  ;  Jung-Tak Oh  ;  David A. LaVan  ;  Joseph P. Vacanti  ;  Jay M. Wilson  ;  Dario O. Fauza 
Citation
 JOURNAL OF PEDIATRIC SURGERY, Vol.41(1) : 34-39, 2006 
Journal Title
JOURNAL OF PEDIATRIC SURGERY
ISSN
 0022-3468 
Issue Date
2006
MeSH
Animals ; Animals, Newborn ; Collagen Type I/analysis ; Elastin/analysis ; Fetus* ; Hernia, Diaphragmatic/surgery* ; Mesoderm/cytology ; Myoblasts ; Sheep ; Tissue Engineering/methods* ; Treatment Outcome
Keywords
Congenital diaphragmatic hernia ; Tissue engineering ; Amniotic fluid ; Skeletal muscle ; Diaphragm ; Congenital anomaliesBirth defects ; Fetus ; Fetal ; Prenatal ; Neonatal
Abstract
PURPOSE: We have previously shown that fetal tissue engineering is a preferred alternative to diaphragmatic repair in a large animal model. This study was aimed at comparing diaphragmatic constructs seeded with mesenchymal amniocytes and fetal myoblasts in this model.
METHODS: Neonatal lambs (n = 14) underwent repair of an experimental diaphragmatic defect with identical scaffolds, either seeded with labeled autologous cells (mesenchymal amniocytes in group 1 and fetal myoblasts in group 2) or as an acellular graft (group 3). At 1 to 12 months postoperatively, implants were harvested for multiple analyses.
RESULTS: Repair failure (reherniation or eventration) was significantly higher in group 3 than in groups 1 and 2, with no difference between groups 1 and 2. Seeded fetal myoblasts quickly lost their myogenic phenotype in vivo. All grafts contained cells with a fibroblastic-myofibroblastic profile. Elastin concentrations and both modular and ultimate tensile strengths were significantly higher in group 1 than in groups 2 and 3. There were no differences in glycosaminoglycans and type I collagen levels among the groups.
CONCLUSIONS: Diaphragmatic repair with a mesenchymal amniocyte-based engineered tendon leads to improved structural outcomes when compared with equivalent fetal myoblast-based and acellular grafts. The amniotic fluid is a preferred cell source for tissue-engineered diaphragmatic reconstruction.
Full Text
http://www.sciencedirect.com/science/article/pii/S002234680500730X
DOI
10.1016/j.jpedsurg.2005.10.011
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Surgery (외과학교실) > 1. Journal Papers
Yonsei Authors
Oh, Jung Tak(오정탁)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/109650
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