Cited 105 times in
Diaphragmatic repair through fetal tissue engineering: a comparison between mesenchymal amniocyte– and myoblast-based constructs
DC Field | Value | Language |
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dc.contributor.author | 오정탁 | - |
dc.date.accessioned | 2015-06-10T12:21:20Z | - |
dc.date.available | 2015-06-10T12:21:20Z | - |
dc.date.issued | 2006 | - |
dc.identifier.issn | 0022-3468 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/109650 | - |
dc.description.abstract | PURPOSE: We have previously shown that fetal tissue engineering is a preferred alternative to diaphragmatic repair in a large animal model. This study was aimed at comparing diaphragmatic constructs seeded with mesenchymal amniocytes and fetal myoblasts in this model. METHODS: Neonatal lambs (n = 14) underwent repair of an experimental diaphragmatic defect with identical scaffolds, either seeded with labeled autologous cells (mesenchymal amniocytes in group 1 and fetal myoblasts in group 2) or as an acellular graft (group 3). At 1 to 12 months postoperatively, implants were harvested for multiple analyses. RESULTS: Repair failure (reherniation or eventration) was significantly higher in group 3 than in groups 1 and 2, with no difference between groups 1 and 2. Seeded fetal myoblasts quickly lost their myogenic phenotype in vivo. All grafts contained cells with a fibroblastic-myofibroblastic profile. Elastin concentrations and both modular and ultimate tensile strengths were significantly higher in group 1 than in groups 2 and 3. There were no differences in glycosaminoglycans and type I collagen levels among the groups. CONCLUSIONS: Diaphragmatic repair with a mesenchymal amniocyte-based engineered tendon leads to improved structural outcomes when compared with equivalent fetal myoblast-based and acellular grafts. The amniotic fluid is a preferred cell source for tissue-engineered diaphragmatic reconstruction. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 34~39 | - |
dc.relation.isPartOf | JOURNAL OF PEDIATRIC SURGERY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Animals, Newborn | - |
dc.subject.MESH | Collagen Type I/analysis | - |
dc.subject.MESH | Elastin/analysis | - |
dc.subject.MESH | Fetus* | - |
dc.subject.MESH | Hernia, Diaphragmatic/surgery* | - |
dc.subject.MESH | Mesoderm/cytology | - |
dc.subject.MESH | Myoblasts | - |
dc.subject.MESH | Sheep | - |
dc.subject.MESH | Tissue Engineering/methods* | - |
dc.subject.MESH | Treatment Outcome | - |
dc.title | Diaphragmatic repair through fetal tissue engineering: a comparison between mesenchymal amniocyte– and myoblast-based constructs | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Surgery (외과학) | - |
dc.contributor.googleauthor | Shaun M. Kunisaki | - |
dc.contributor.googleauthor | Julie R. Fuchs | - |
dc.contributor.googleauthor | Amir Kaviani | - |
dc.contributor.googleauthor | Jung-Tak Oh | - |
dc.contributor.googleauthor | David A. LaVan | - |
dc.contributor.googleauthor | Joseph P. Vacanti | - |
dc.contributor.googleauthor | Jay M. Wilson | - |
dc.contributor.googleauthor | Dario O. Fauza | - |
dc.identifier.doi | 10.1016/j.jpedsurg.2005.10.011 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A02397 | - |
dc.relation.journalcode | J01689 | - |
dc.identifier.eissn | 1531-5037 | - |
dc.identifier.pmid | 16410104 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S002234680500730X | - |
dc.subject.keyword | Congenital diaphragmatic hernia | - |
dc.subject.keyword | Tissue engineering | - |
dc.subject.keyword | Amniotic fluid | - |
dc.subject.keyword | Skeletal muscle | - |
dc.subject.keyword | Diaphragm | - |
dc.subject.keyword | Congenital anomaliesBirth defects | - |
dc.subject.keyword | Fetus | - |
dc.subject.keyword | Fetal | - |
dc.subject.keyword | Prenatal | - |
dc.subject.keyword | Neonatal | - |
dc.contributor.alternativeName | Oh, Jung Tak | - |
dc.contributor.affiliatedAuthor | Oh, Jung Tak | - |
dc.contributor.affiliatedAuthor | 오정탁 | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 41 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 34 | - |
dc.citation.endPage | 39 | - |
dc.identifier.bibliographicCitation | JOURNAL OF PEDIATRIC SURGERY, Vol.41(1) : 34-39, 2006 | - |
dc.identifier.rimsid | 38895 | - |
dc.type.rims | ART | - |
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