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Construction of Functional Soft Tissues From Premodulated Smooth Muscle Cells Using a Bioreactor System

Authors
 Jae Min Cha  ;  Si-Nae Park  ;  Guen-Oh Park  ;  Jeong Koo Kim  ;  Hwal Suh 
Citation
 ARTIFICIAL ORGANS, Vol.30(9) : 704-707, 2006 
Journal Title
 ARTIFICIAL ORGANS 
ISSN
 0160-564X 
Issue Date
2006
MeSH
Actins/metabolism* ; Animals ; Bioreactors* ; Cell Culture Techniques ; Cell Differentiation* ; Muscle, Smooth/physiology* ; Myocytes, Smooth Muscle/cytology* ; Rabbits ; Stress, Mechanical ; Time Factors ; Tissue Engineering/instrumentation* ; Tissue Engineering/methods ; Substance
Keywords
Smooth muscle tissues ; Cyclic mechanical strain ; Bioreactor ; Mechanical properties
Abstract
Artificial smooth muscle tissues should be constructed with well-differentiated and aligned smooth muscle cells (SMCs) for proper functioning. In a previous study, we produced cell/scaffold hybrids composed of consistently aligned SMCs in a contractile state using cyclic mechanical strain. In this study, the preconditioned hybrids were organized as functional smooth muscle constructs, which had a high cellular density, using a bioreactor system. We determined that the alignment and contractile phenotype of the initially generated SMCs would be retained after a 7-day culture period in a bioreactor. Mechanical properties of the smooth muscle constructs were measured and compared with those of native smooth muscle tissues and acellular scaffolds. The constructs had a denser cell concentration than the preconditioned hybrids, although they were not fully filled with cells. The premodulated cell alignment and contractile phenotype were retained after culture in a bioreactor. The 7-day-cultured constructs had similar allowed stress levels to native tissues while their stiffness was much lower, suggesting that they had malleable and durable characteristics. These results suggest that functional smooth muscle tissues with mechanical stability can be produced using premodulated SMCs and a bioreactor system.
Full Text
http://onlinelibrary.wiley.com/doi/10.1111/j.1525-1594.2006.00287.x/abstract
DOI
10.1111/j.1525-1594.2006.00287.x
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Medical Engineering (의학공학교실) > 1. Journal Papers
Yonsei Authors
Park, Si Nae(박시내)
Suh, Hwal(서활)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/109625
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