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장기간 고포도당으로 자극한 배양 족세포에서 안지오텐신 2 수용체 차단제에 의한 세포비후 차단과 세포주기 조절 단백의 차등 발현

Other Titles
 Angiotensin II Receptor Blocker Induced Inhibition of Cellular Hypertrophy and Differential Expression of Cyclin-dependent Kinase Inhibitors in Cultured Podocytes Stimulated by Long-term High Glucose 
Authors
 박형천  ;  허종호  ;  류동열  ;  유태현  ;  정동섭  ;  김진주  ;  곽승재  ;  이금희  ;  한대석  ;  강신욱 
Citation
 Korean Journal of Nephrology (대한신장학회지), Vol.25(5) : 695-704, 2006 
Journal Title
Korean Journal of Nephrology(대한신장학회지)
ISSN
 1225-0015 
Issue Date
2006
Keywords
Podocyte ; p27Kip1 ; High glucose ; Angiotensin II receptor blocker
Abstract
Background : Hypertrophy of podocytes is observed in type 2 diabetic patients. Cellular hypertrophy requires combined effects of various mitogeninduced entry into the cell cycle and subsequent cell cycle arrest at the G1/S interphase. This cell cycle arrest is mediated by various cyclin-dependent kinase inhibitors (CKIs). We investigated the effect of angiotensin II receptor blocker (ARB) treatment on podocyte hypertrophy and CKIs expression in cultured podocytes stimulated by long-term high glucose.
Methods : Immortalized mouse podocytes were cultured in media containing 5.6 mM normal glucose (NG), 30 mM high glucose (HG), or NG+angiotensin II (AII, 10(-7)M) for 7 days with or without ARB (L-158,809, 10(-6)M). Cellular hypertrophy was assessed by measurement of cellular protein/cell counts, and CKIs mRNA and protein expression were assessed by reverse-transcription polymerase chain reaction (RT-PCR) and Western blot, respectively.
Results : Cellular hypertrophy was induced in podocytes exposed to HG or AII compared to NG cells and this HG-induced cellular hypertrophy was inhibited with ARB treatment by 70% (p<0.05). In addition, there were 1.5-fold and 2.0 fold increases in p27(Kip1) mRNA and protein expression, respectively, in HG-stimulated podocytes compared to NG-treated cells (p<0.05). p27(Kip1) mRNA and protein expression were also increased in cultured podocytes stimulated by AII by 156% and 199%, respectively (p<0.05). ARB treatment ameliorated HG-induced increase in p27(Kip1) mRNA by 75% and protein expression by 70% (p<0.05). In contrast, there were no significant changes in p21(Cip1) and p57(Kip2) protein expression in cultured podocytes exposed to HG or AII.
Conclusion : High glucose induced significant cellular hypertrophy and increased p27(Kip1) mRNA and protein expression in cultured mouse podocytes, and these changes were effectively inhibited by ARB treatment.
Files in This Item:
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Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Kang, Shin Wook(강신욱) ORCID logo https://orcid.org/0000-0002-5677-4756
Park, Hyeong Cheon(박형천) ORCID logo https://orcid.org/0000-0002-1550-0812
Han, Dae Suk(한대석)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/109415
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