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장기간 고포도당으로 자극한 배양 족세포에서 안지오텐신 2 수용체 차단제에 의한 세포비후 차단과 세포주기 조절 단백의 차등 발현
DC Field | Value | Language |
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dc.contributor.author | 강신욱 | - |
dc.contributor.author | 박형천 | - |
dc.contributor.author | 한대석 | - |
dc.date.accessioned | 2015-06-10T12:13:32Z | - |
dc.date.available | 2015-06-10T12:13:32Z | - |
dc.date.issued | 2006 | - |
dc.identifier.issn | 1225-0015 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/109415 | - |
dc.description.abstract | Background : Hypertrophy of podocytes is observed in type 2 diabetic patients. Cellular hypertrophy requires combined effects of various mitogeninduced entry into the cell cycle and subsequent cell cycle arrest at the G1/S interphase. This cell cycle arrest is mediated by various cyclin-dependent kinase inhibitors (CKIs). We investigated the effect of angiotensin II receptor blocker (ARB) treatment on podocyte hypertrophy and CKIs expression in cultured podocytes stimulated by long-term high glucose. Methods : Immortalized mouse podocytes were cultured in media containing 5.6 mM normal glucose (NG), 30 mM high glucose (HG), or NG+angiotensin II (AII, 10(-7)M) for 7 days with or without ARB (L-158,809, 10(-6)M). Cellular hypertrophy was assessed by measurement of cellular protein/cell counts, and CKIs mRNA and protein expression were assessed by reverse-transcription polymerase chain reaction (RT-PCR) and Western blot, respectively. Results : Cellular hypertrophy was induced in podocytes exposed to HG or AII compared to NG cells and this HG-induced cellular hypertrophy was inhibited with ARB treatment by 70% (p<0.05). In addition, there were 1.5-fold and 2.0 fold increases in p27(Kip1) mRNA and protein expression, respectively, in HG-stimulated podocytes compared to NG-treated cells (p<0.05). p27(Kip1) mRNA and protein expression were also increased in cultured podocytes stimulated by AII by 156% and 199%, respectively (p<0.05). ARB treatment ameliorated HG-induced increase in p27(Kip1) mRNA by 75% and protein expression by 70% (p<0.05). In contrast, there were no significant changes in p21(Cip1) and p57(Kip2) protein expression in cultured podocytes exposed to HG or AII. Conclusion : High glucose induced significant cellular hypertrophy and increased p27(Kip1) mRNA and protein expression in cultured mouse podocytes, and these changes were effectively inhibited by ARB treatment. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 695~704 | - |
dc.relation.isPartOf | Korean Journal of Nephrology (대한신장학회지) | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | 장기간 고포도당으로 자극한 배양 족세포에서 안지오텐신 2 수용체 차단제에 의한 세포비후 차단과 세포주기 조절 단백의 차등 발현 | - |
dc.title.alternative | Angiotensin II Receptor Blocker Induced Inhibition of Cellular Hypertrophy and Differential Expression of Cyclin-dependent Kinase Inhibitors in Cultured Podocytes Stimulated by Long-term High Glucose | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학) | - |
dc.contributor.googleauthor | 박형천 | - |
dc.contributor.googleauthor | 허종호 | - |
dc.contributor.googleauthor | 류동열 | - |
dc.contributor.googleauthor | 유태현 | - |
dc.contributor.googleauthor | 정동섭 | - |
dc.contributor.googleauthor | 김진주 | - |
dc.contributor.googleauthor | 곽승재 | - |
dc.contributor.googleauthor | 이금희 | - |
dc.contributor.googleauthor | 한대석 | - |
dc.contributor.googleauthor | 강신욱 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00053 | - |
dc.contributor.localId | A01759 | - |
dc.contributor.localId | A04272 | - |
dc.relation.journalcode | J02066 | - |
dc.subject.keyword | Podocyte | - |
dc.subject.keyword | p27Kip1 | - |
dc.subject.keyword | High glucose | - |
dc.subject.keyword | Angiotensin II receptor blocker | - |
dc.contributor.alternativeName | Kang, Shin Wook | - |
dc.contributor.alternativeName | Park, Hyeong Cheon | - |
dc.contributor.alternativeName | Han, Dae Suk | - |
dc.contributor.affiliatedAuthor | Kang, Shin Wook | - |
dc.contributor.affiliatedAuthor | Park, Hyeong Cheon | - |
dc.contributor.affiliatedAuthor | Han, Dae Suk | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 25 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 695 | - |
dc.citation.endPage | 704 | - |
dc.identifier.bibliographicCitation | Korean Journal of Nephrology (대한신장학회지), Vol.25(5) : 695-704, 2006 | - |
dc.identifier.rimsid | 57370 | - |
dc.type.rims | ART | - |
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