Animals ; Blotting, Western ; Central Nervous System Depressants/adverse effects* ; Central Nervous System Depressants/blood ; Cyanamide/pharmacology ; Ethanol/adverse effects* ; Ethanol/blood ; Hypoglycemic Agents/pharmacology* ; Immunoblotting ; Interpersonal Relations ; Male ; Mitochondria, Liver/drug effects ; Mitochondria, Liver/enzymology ; Rats ; Rats, Sprague-Dawley ; Reverse Transcriptase Polymerase Chain Reaction ; Rosiglitazone ; Social Behavior ; Thiazolidinediones/pharmacology* ; Up-Regulation/drug effects
Keywords
ethanol ; rosiglitazone ; acetaldehyde ; western blotting ; mitochondria ; cyanamide ; rats, sprague-dawley ; rats ; hangover from alcohol ; social interaction
Abstract
Aims: To assess the efficacy of rosiglitazone in blocking ethanol-induced hangover in rats. Methods: Rats injected with ethanol (4 g/kg body weight) were subjected to social interaction tests. Levels of aldehyde dehydrogenase 2 (ALD2), involved in an anti-hangover mechanism, were measured by semi-quantitative RT–PCR and western blot analysis. Results: Rosiglitazone caused an upregulation of mitochondrial ALD2, thus significantly detoxifying acetaldehyde. Conclusions: Rosiglitazone alleviated the symptoms of ethanol-induced hangover by inducing ALD2 expression; this result was reconfirmed by eliminating the effect of rosiglitazone by injecting cyanamide, an ALD2 inhibitor.