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Accumulation of β-catenin protein, mutations in exon-3 of the β-catenin gene and a loss of heterozygosity of 5q22 in solid pseudopapillary tumor of the pancreas

Authors
 Seong Min Kim  ;  Chuan Dong Sun  ;  Ki Chung Park  ;  Ho Guen Kim  ;  Woo Jung Lee  ;  Seung Hoon Choi 
Citation
 JOURNAL OF SURGICAL ONCOLOGY, Vol.94(5) : 418-425, 2006 
Journal Title
 JOURNAL OF SURGICAL ONCOLOGY 
ISSN
 0022-4790 
Issue Date
2006
MeSH
Adolescent ; Adult ; Carcinoma, Papillary/genetics* ; Carcinoma, Papillary/pathology ; Child ; Chromosomes, Human, Pair 5/genetics* ; Cystadenoma, Papillary ; DNA Mutational Analysis ; Exons/genetics* ; Female ; Gene Expression ; Humans ; Immunohistochemistry ; Loss of Heterozygosity* ; Male ; Microsatellite Repeats ; Middle Aged ; Mutation* ; Pancreatic Neoplasms/genetics* ; Pancreatic Neoplasms/pathology ; beta Catenin/genetics*
Keywords
solid pseudopapillary tumor ; pancreas ; mutation ; immunohistochemistry ; loss of heterozygosity
Abstract
BACKGROUND: Solid pseudopapillary tumors (SPT) of the pancreas are neoplasms with a low malignant potential. The molecular events contributing to the pathogenesis of SPTs are still unknown. OBJECTIVES: This study was intended to help better understand the early steps of human SPT development. METHODS: We microdissected 20 SPTs and normal pancreatic tissue. In addition, we examined the DNA from each SPT for mutations in exon-3 of beta-catenin and loss of heterozygosity (LOH) on 9 chromosome arms using 10 microsatellite markers. Immunohistochemical staining for beta-catenin was performed. RESULTS: Activating mutations between codons 32 and 37 of beta-catenin exon-3 were present in 16 cases (80%). Allelic loss on chromosome 5q22.1 was present in 10 cases (55.5%), while no allelic loss was found on chromosomes 1p, 6q, 9p, 9q, 11p, 11q, 17p, or 22q. Nuclear accumulation of beta-catenin was found in 20 cases (100%). CONCLUSION: Mutations in exon-3 of the beta-catenin gene, nuclear accumulation of beta-catenin, and LOH on chromosome 5q22.1 in SPT tissue suggest that these mutations are involved in SPT tumorigenesis.
Full Text
http://onlinelibrary.wiley.com/doi/10.1002/jso.20509/abstract
DOI
10.1002/jso.20509
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Surgery (외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
Yonsei Authors
Kim, Ho Keun(김호근)
Park, Ki Cheong(박기청) ORCID logo https://orcid.org/0000-0002-3435-3985
Lee, Woo Jung(이우정) ORCID logo https://orcid.org/0000-0001-9273-261X
Choi, Seung Hoon(최승훈)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/109176
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