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Combined Sustained Delivery of Basic Fibroblast Growth Factor and Administration of Granulocyte Colony-Stimulationg Factor

Authors
 Oju Jeon  ;  Ki-Chul Hwang  ;  Kyung-Jong Yoo  ;  Byung-Soo Kim 
Citation
 JOURNAL OF ENDOVASCULAR THERAPY, Vol.13(2) : 175-181, 2006 
Journal Title
 JOURNAL OF ENDOVASCULAR THERAPY 
ISSN
 1526-6028 
Issue Date
2006
MeSH
Animals ; Disease Models, Animal ; Drug Synergism ; Enzyme-Linked Immunosorbent Assay ; Female ; Fibroblast Growth Factor 2/administration & dosage* ; Fibroblast Growth Factor 2/pharmacology ; Flow Cytometry ; Granulocyte Colony-Stimulating Factor/administration & dosage* ; Granulocyte Colony-Stimulating Factor/pharmacology ; Hindlimb/blood supply* ; Ischemia/drug therapy* ; Mice ; Mice, Inbred C57BL ; Neovascularization, Physiologic/drug effects* ; Random Allocation ; Recombinant Proteins
Keywords
mouse model ; limb ischemia model ; angiogenic therapy ; basic fibroblast growth factor ; endothelial progenitor cell mobilization ; granulocyte colony-stimulating factor
Abstract
Purpose: To investigate whether the efficacy of a single angiogenic therapy (sustained delivery of basic fibroblast growth factor [bFGF] or administration of granulocyte colony-stimulating factor [G-CSF]) can be enhanced further by combining the therapies. Methods: One day after surgical induction of hind-limb ischemia, groups of 6 mice were randomized to receive either no treatment, sustained delivery (SD) of bFGF, endothelial progenitor cell (EPC) mobilization with G-CSF administration, or a combination of bFGF SD+G-CSF administration. Results: G-CSF administration increased significantly (p<0.05) the number of EPC lineages (CD34+/AC133+ cells) in both peripheral blood and bone marrow compared to no G-CSF administration. The bFGF SD and G-CSF administration individually increased the capillary and arteriole densities significantly versus no treatment (capillary density: 659±48/mm2 and 385±59/mm2, respectively, versus 280±28/mm2; p<0.05; arteriole density: 34±9/mm2 and 41±6/mm2, respectively, versus 15±2/mm2; p<0.05). Importantly, bFGF SD+G-CSF further increased the capillary and arteriole densities compared to either strategy alone (capillary density: 786±40/mm2 versus 659±48/mm2 and 385±59/mm2, respectively, p<0.05; arteriole density: 55±10/mm2 versus 34±9/mm2 and 41±6/mm2, respectively, p<0.05). Conclusion: This study demonstrates that the combined therapy of sustained bFGF delivery and G-CSF administration potentiates the angiogenic efficacy of either single therapy in mouse hind-limb ischemia models.
Full Text
http://jevtonline.org/doi/abs/10.1583/05-1713MR.1
DOI
10.1583/05-1713MR.1
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Thoracic and Cardiovascular Surgery (흉부외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
Yonsei Authors
Yoo, Kyung Jong(유경종) ORCID logo https://orcid.org/0000-0002-9858-140X
Hwang, Ki Chul(황기철)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/109085
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