2 211

Cited 86 times in

Activation of Spinal GABA Receptors Attenuates Chronic Central Neuropathic Pain after Spinal Cord Injury

Authors
 Young Seob Gwak  ;  Huai Yu Tan  ;  Taick Sang Nam  ;  Kwang Se Paik  ;  Claire E. Hulsebosch  ;  Joong Woo Leem 
Citation
 JOURNAL OF NEUROTRAUMA, Vol.23(7) : 1111-1124, 2006 
Journal Title
 JOURNAL OF NEUROTRAUMA 
ISSN
 0897-7151 
Issue Date
2006
MeSH
Animals ; GABA Antagonists/pharmacology ; Male ; Pain/etiology ; Pain/metabolism* ; Pain/prevention & control ; Pain Measurement/drug effects ; Pain Measurement/methods ; Rats ; Rats, Sprague-Dawley ; Receptors, GABA/metabolism* ; Spinal Cord/drug effects ; Spinal Cord/metabolism* ; Spinal Cord Injuries/complications ; Spinal Cord Injuries/metabolism*
Keywords
baclofen ; central neuropathic pain ; Hemitransection ; muscimol ; spinal cord injury ; wide dynamic range neuron
Abstract
In this study, we investigated the role of the spinal GABAergic system in central neuropathic painlike outcomes following spinal cord injury (SCI) produced by a spinal hemitransection at T13 of the rat. After SCI, mechanical allodynia develops bilaterally in both hind paws of the rat, lasting longer than 40 days, as evidenced by an increase in paw withdrawal frequency in response to a weak von Frey filament. In naive rats, intrathecal (i.t.) administration in the lumbar spinal cord of GABAA and GABAB receptor antagonists, bicuculline (1-5 microg) and phaclofen (0.1-5 microg), respectively, causes a dose-dependent increase in the magnitude of mechanical allodynia. The SCI-induced mechanical allodynia in both hind-paws is attenuated by i.t. administration in the lumbar spinal cord of GABAA or GABAB receptor agonists, muscimol (1 microg) or baclofen (0.5 microg), respectively. In electrophysiological experiments, rats with SCI show a bilateral increase in hyperexcitability in response to natural stimuli in wide dynamic range (WDR) neurons in the lumbar spinal dorsal horn. The topical application of muscimol (1 microg) or baclofen (0.5 microg) onto the lumbar cord surface reduce the SCIinduced increased responsiveness of WDR neurons. Inhibitory effects of muscimol and baclofen on both the behavioral mechanical allodynia and the hyperexcitability in WDR neuron with SCI compared to controls, were antagonized by pre-treatment of bicuculline (10 microg) and phaclofen (5 microg), respectively. This study provides behavioral and electrophysiological evidence for the important role of the loss of spinal inhibitory tone, mediated by activation of both GABAA and GABAB receptors, in the development of central neuropathic pain following SCI.
Full Text
http://online.liebertpub.com/doi/abs/10.1089/neu.2006.23.1111
DOI
10.1089/neu.2006.23.1111
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Physiology (생리학교실) > 1. Journal Papers
Yonsei Authors
Nam, Taick Sang(남택상)
Paik, Kwang Se(백광세)
Leem, Joong Woo(임중우) ORCID logo https://orcid.org/0000-0002-1605-2230
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/109028
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse