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Comparative Proteomics of Ovarian Epithelial Tumors

 Hee Jung An  ;  Dong Su Kim  ;  Yong Kyu Park  ;  Sei Kwang Kim  ;  Yoon Pyo Choi  ;  Suki Kang  ;  Boxiao Ding  ;  Nam Hoon Cho 
 JOURNAL OF PROTEOME RESEARCH, Vol.5(5) : 1082-1090, 2006 
Journal Title
Issue Date
Adult ; Biomarkers, Tumor/analysis ; Biomarkers, Tumor/metabolism ; Carcinoma in Situ/metabolism ; Carcinoma in Situ/pathology ; Cluster Analysis ; Epithelial Cells/metabolism ; Epithelial Cells/pathology ; Female ; Humans ; Middle Aged ; Models, Biological ; Neoplasms, Glandular and Epithelial/metabolism* ; Neoplasms, Glandular and Epithelial/pathology ; Ovarian Neoplasms/metabolism* ; Ovarian Neoplasms/pathology* ; Proteomics/methods* ; Reference Values ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods
ovarian tumor ; mucinous tumor ; low malignant potential ; proteomics ; distance map tree
We analyzed 12 ovarian epithelial tumors using 2D PAGE-based comparative proteomics to construct intra- and inter-tumoral distance map trees and to discover surrogate biomarkers indicative of an ovarian tumor. The analysis was performed after laser microdissection of 12 fresh-frozen tissue samples, including 4 serous, 5 mucinous, and 3 endometrioid tumors, with correlation with their histopathological characteristics. Ovarian epithelial tumors and normal tissues showed an apparent separation on the distance map tree. Mucinous carcinomas were closest to the normal group, whereas serous carcinomas were located furthest from the normal group. All mucinous tumors with aggressive histology were separated from the low malignant potential (LMP) group. The benign-looking cysts adjacent to the intraepithelial carcinoma (IEC) showed an expression pattern identical to that of the IEC area. The extent of change on the lineages leading to the mucinous and serous carcinoma was 1.98-fold different. The overall gene expression profiles of serous or endometrioid carcinomas appeared to be less affected by grade or stage than by histologic type. The potential candidate biomarkers screened in ovarian tumors and found to be significantly up-regulated in comparison to normal tissues were as follows:  NM23, annexin-1, protein phosphatase-1, ferritin light chain, proteasome α-6, and NAGK (N-acetyl glucosamine kinase). In conclusion, ovarian mucinous tumors are distinct from other ovarian epithelial tumors. LMP mucinous tumors showing histologically aggressive features belong to mucinous carcinoma on the proteomic basis.
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1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Obstetrics and Gynecology (산부인과학교실) > 1. Journal Papers
Yonsei Authors
Kang, Suki(강숙희) ORCID logo https://orcid.org/0000-0002-9957-3479
Kim, Sei Kwang(김세광)
Cho, Nam Hoon(조남훈) ORCID logo https://orcid.org/0000-0002-0045-6441
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