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Inhibitory effects of epigallocatechin-3-O-gallate on serum-stimulated rat aortic smooth muscle cells via nuclear factor-κB down-modulation

Authors
 Dong-Wook Han  ;  Hye Ryeon Lim  ;  Hyun Sook Baek  ;  Mi Hee Lee  ;  Seung Jin Lee  ;  Suong-Hyu Hyon  ;  Jong-Chul Park 
Citation
 BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Vol.345(1) : 148-155, 2006 
Journal Title
 BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 
ISSN
 0006-291X 
Issue Date
2006
MeSH
Animals ; Antioxidants/administration & dosage ; Aorta/cytology ; Aorta/drug effects ; Aorta/metabolism* ; Catechin/administration & dosage ; Catechin/analogs & derivatives* ; Cell Adhesion/drug effects ; Cell Movement/drug effects ; Cell Proliferation/drug effects ; Cells, Cultured ; Dose-Response Relationship, Drug ; Down-Regulation/drug effects ; Down-Regulation/physiology ; Myocytes, Smooth Muscle/drug effects ; Myocytes, Smooth Muscle/metabolism* ; NF-kappa B/metabolism* ; Rats ; Serum/metabolism
Keywords
Epigallocatechin-3-O-gallate ; Antiproliferation ; Rat aortic smooth muscle cell ; Cell cycle arrest ; Nuclear factor-κB
Abstract
The abnormal growth of vascular smooth muscle cells (VSMCs) plays an important role in vascular diseases, including atherosclerosis and restenosis after angioplasty. Although (−)-epigallocatechin-3-O-gallate (EGCG) has antiproliferative effects on various cells, relatively a little is known about precise mechanisms of the inhibitory effects of EGCG on SMCs. In this study, the inhibitory effects of EGCG on attachment, proliferation, migration, and cell cycle of rat aortic SMCs (RASMCs) with serum stimulation were investigated. Also, the involvement of nuclear factor-κB (NF-κB) during these inhibitions by EGCG was examined. EGCG treatment resulted in significant (p < 0.05) inhibition in attachment and proliferation of RASMCs induced by serum. While non-treated RASMCs migrated into denuded area in response to serum and showed essentially complete closure after 36 h, EGCG-treated cells covered only 31% of the area even after 48 h of incubation. Furthermore, EGCG treatment resulted in an appreciable cell cycle arrest at both G0/G1- and G2/M-phases. The immunoblot analysis revealed that the constitutive expression of NF-κB/p65 nuclear protein in RASMCs was lowered by EGCG in both the cytosol and the nucleus in a dose-dependent manner. These results suggest that the EGCG-caused inhibitory effects on RASMCs may be mediated through NF-κB down-modulation.
Full Text
http://www.sciencedirect.com/science/article/pii/S0006291X06009132
DOI
10.1016/j.bbrc.2006.04.072
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Medical Engineering (의학공학교실) > 1. Journal Papers
Yonsei Authors
Park, Jong Chul(박종철) ORCID logo https://orcid.org/0000-0003-0083-5991
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/108831
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