2 463

Cited 4 times in

Cloning and characterization of the 5′-flanking region of the Ehox gene

Authors
 Woon Kyu Lee  ;  Yong-Man Kim  ;  Nasir Malik  ;  Chang Ma  ;  Heiner Westphal 
Citation
 BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Vol.341(1) : 225-231, 2006 
Journal Title
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
ISSN
 0006-291X 
Issue Date
2006
MeSH
5' Flanking Region/genetics* ; Animals ; Base Sequence ; CCAAT-Binding Factor/genetics* ; Cloning, Molecular ; Homeodomain Proteins/genetics* ; Mice ; Molecular Sequence Data ; Promoter Regions, Genetic/genetics ; Transcription Factors/genetics* ; Transcriptional Activation/genetics*
Keywords
Ehox ; Nuclear factor Y ; 5′-Flanking
Abstract
The paired-like homeobox-containing gene Ehox plays a role in embryonic stem cell differentiation and is highly expressed in the developing placenta and thymus. To understand the mechanisms of regulation of Ehox gene expression, the 5′-flanking region of the Ehox gene was isolated from a mouse BAC library. 5′-RACE analysis revealed a single transcriptional start site 130 nucleotides upstream of the translation initiation codon. Transient transfection with a luciferase reporter gene under the control of serially deleted 5′-flanking sequences revealed that the nt −84 to −68 region contained a positive cis-acting element for efficient expression of the Ehox gene. Mutational analysis of this region and oligonucleotide competition in the electrophoretic mobility shift assay revealed the presence of a CCAAT box, which is a target for transcription nuclear factor Y (NFY). NFY is essential for positive gene regulation. No tissue-specific enhancer was identified in the 1.9-kb 5′-flanking region of the Ehox gene. Ehox is expressed during the early stages of embryo development, specifically in the brain at 9.5 dpc, as well as during the late stages of embryo development. These results suggest that NFY is an essential regulatory factor for Ehox transcriptional activity, which is important for the post-implantation stage of the developing embryo.
Full Text
http://www.sciencedirect.com/science/article/pii/S0006291X05029359
DOI
10.1016/j.bbrc.2005.12.176
Appears in Collections:
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
Yonsei Authors
Lee, Woon Kyu(이운규)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/108785
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse

Links