Cited 14 times in
Induced expression of insulin-like growth factor-1 by amniotic membrane-conditioned medium in cultured human corneal epithelial cells
DC Field | Value | Language |
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dc.contributor.author | 이준행 | - |
dc.contributor.author | 이형근 | - |
dc.contributor.author | 홍순원 | - |
dc.contributor.author | 김응권 | - |
dc.contributor.author | 이종은 | - |
dc.date.accessioned | 2015-06-10T11:51:42Z | - |
dc.date.available | 2015-06-10T11:51:42Z | - |
dc.date.issued | 2006 | - |
dc.identifier.issn | 0146-0404 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/108754 | - |
dc.description.abstract | To determine the effect of amniotic membrane-conditioned medium (AMCM), via insulin-like growth factor (IGF)-1 induction, on human corneal epithelial cell (HCEC) proliferation. METHODS: HCECs were cultured from corneal limbal tissue with supplemented hormonal epithelial medium (SHEM). After administration of AMCM, cell proliferation was evaluated with an MTT assay and DNA synthesis with methyl-[(3)H]-thymidine incorporation assay. RT-PCR and Western immunoblot analyses were performed, to determine potential inducible factors that may be associated with AMCM-induced cell proliferation. Neutralizing anti-IGF-1 antibody and small interfering (si)RNA were also used to clarify the role of IGF-1 in AMCM-induced HCEC proliferation. RESULTS: HCEC proliferation increased after AMCM treatment. Of the cytokines known to be associated with HCEC proliferation, only IGF-1 expression was upregulated in response to AMCM in a dose- and time-dependent manner. The IGF-1 induction effect was found on both AMCM from live AM and from cryopreserved AM. HCEC proliferation was also increased by addition of exogenous IGF-1. AMCM-induced HCEC proliferation was inhibited in the presence of neutralizing anti-IGF-1 antibody and IGF-1 siRNA. Finally, Akt phosphorylation was increased in HCECs after AMCM treatment and was inhibited by IGF-1 siRNA. CONCLUSIONS: IGF-1 is induced by AMCM during HCEC proliferation, and this induction may play an important role in the amniotic membrane during HCEC proliferation and migration in several intractable corneal epithelial defects. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 864~872 | - |
dc.relation.isPartOf | INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Amnion/physiology* | - |
dc.subject.MESH | Blotting, Western | - |
dc.subject.MESH | Cell Proliferation/drug effects | - |
dc.subject.MESH | Cells, Cultured | - |
dc.subject.MESH | Culture Media, Conditioned/pharmacology* | - |
dc.subject.MESH | DNA/biosynthesis | - |
dc.subject.MESH | Dose-Response Relationship, Drug | - |
dc.subject.MESH | Epithelium, Corneal/cytology* | - |
dc.subject.MESH | Epithelium, Corneal/metabolism* | - |
dc.subject.MESH | Female | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Insulin-Like Growth Factor I/biosynthesis* | - |
dc.subject.MESH | Insulin-Like Growth Factor I/genetics | - |
dc.subject.MESH | Phosphorylation | - |
dc.subject.MESH | Proto-Oncogene Proteins c-akt/metabolism | - |
dc.subject.MESH | RNA, Messenger/metabolism | - |
dc.subject.MESH | RNA, Small Interfering/pharmacology | - |
dc.subject.MESH | Reverse Transcriptase Polymerase Chain Reaction | - |
dc.subject.MESH | Tetrazolium Salts | - |
dc.subject.MESH | Thiazoles | - |
dc.subject.MESH | Time Factors | - |
dc.subject.MESH | Up-Regulation | - |
dc.title | Induced expression of insulin-like growth factor-1 by amniotic membrane-conditioned medium in cultured human corneal epithelial cells | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Ophthalmology (안과학) | - |
dc.contributor.googleauthor | Joon H. Lee | - |
dc.contributor.googleauthor | Ik Hee Ryu | - |
dc.contributor.googleauthor | Eung Kweon Kim | - |
dc.contributor.googleauthor | Jong Eun Lee | - |
dc.contributor.googleauthor | SoonWon Hong | - |
dc.contributor.googleauthor | Hyung Keun Lee | - |
dc.identifier.doi | 10.1167/iovs.05-0596 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A03180 | - |
dc.contributor.localId | A03303 | - |
dc.contributor.localId | A04411 | - |
dc.contributor.localId | A00831 | - |
dc.contributor.localId | A03146 | - |
dc.relation.journalcode | J01187 | - |
dc.identifier.eissn | 1552-5783 | - |
dc.identifier.pmid | 16505018 | - |
dc.contributor.alternativeName | Lee, Joon Haeng | - |
dc.contributor.alternativeName | Lee, Hyung Keun | - |
dc.contributor.alternativeName | Hong, Soon Won | - |
dc.contributor.alternativeName | Kim, Eung Kweon | - |
dc.contributor.alternativeName | Lee, Jong Eun | - |
dc.contributor.affiliatedAuthor | Lee, Joon Haeng | - |
dc.contributor.affiliatedAuthor | Lee, Hyung Keun | - |
dc.contributor.affiliatedAuthor | Hong, Soon Won | - |
dc.contributor.affiliatedAuthor | Kim, Eung Kweon | - |
dc.contributor.affiliatedAuthor | Lee, Jong Eun | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 47 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 864 | - |
dc.citation.endPage | 872 | - |
dc.identifier.bibliographicCitation | INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, Vol.47(3) : 864-872, 2006 | - |
dc.identifier.rimsid | 57681 | - |
dc.type.rims | ART | - |
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