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Induced expression of insulin-like growth factor-1 by amniotic membrane-conditioned medium in cultured human corneal epithelial cells

DC FieldValueLanguage
dc.contributor.author이준행-
dc.contributor.author이형근-
dc.contributor.author홍순원-
dc.contributor.author김응권-
dc.contributor.author이종은-
dc.date.accessioned2015-06-10T11:51:42Z-
dc.date.available2015-06-10T11:51:42Z-
dc.date.issued2006-
dc.identifier.issn0146-0404-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/108754-
dc.description.abstractTo determine the effect of amniotic membrane-conditioned medium (AMCM), via insulin-like growth factor (IGF)-1 induction, on human corneal epithelial cell (HCEC) proliferation. METHODS: HCECs were cultured from corneal limbal tissue with supplemented hormonal epithelial medium (SHEM). After administration of AMCM, cell proliferation was evaluated with an MTT assay and DNA synthesis with methyl-[(3)H]-thymidine incorporation assay. RT-PCR and Western immunoblot analyses were performed, to determine potential inducible factors that may be associated with AMCM-induced cell proliferation. Neutralizing anti-IGF-1 antibody and small interfering (si)RNA were also used to clarify the role of IGF-1 in AMCM-induced HCEC proliferation. RESULTS: HCEC proliferation increased after AMCM treatment. Of the cytokines known to be associated with HCEC proliferation, only IGF-1 expression was upregulated in response to AMCM in a dose- and time-dependent manner. The IGF-1 induction effect was found on both AMCM from live AM and from cryopreserved AM. HCEC proliferation was also increased by addition of exogenous IGF-1. AMCM-induced HCEC proliferation was inhibited in the presence of neutralizing anti-IGF-1 antibody and IGF-1 siRNA. Finally, Akt phosphorylation was increased in HCECs after AMCM treatment and was inhibited by IGF-1 siRNA. CONCLUSIONS: IGF-1 is induced by AMCM during HCEC proliferation, and this induction may play an important role in the amniotic membrane during HCEC proliferation and migration in several intractable corneal epithelial defects.-
dc.description.statementOfResponsibilityopen-
dc.format.extent864~872-
dc.relation.isPartOfInvestigative Ophthalmology & Visual Science-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.titleInduced expression of insulin-like growth factor-1 by amniotic membrane-conditioned medium in cultured human corneal epithelial cells-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Ophthalmology (안과학)-
dc.contributor.googleauthorJoon H. Lee-
dc.contributor.googleauthorIk Hee Ryu-
dc.contributor.googleauthorEung Kweon Kim-
dc.contributor.googleauthorJong Eun Lee-
dc.contributor.googleauthorSoonWon Hong-
dc.contributor.googleauthorHyung Keun Lee-
dc.identifier.doi10.1167/iovs.05-0596-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA03180-
dc.contributor.localIdA03303-
dc.contributor.localIdA04411-
dc.contributor.localIdA00831-
dc.contributor.localIdA03146-
dc.relation.journalcodeJ01187-
dc.contributor.alternativeNameLee, Joon Haeng-
dc.contributor.alternativeNameLee, Hyung Keun-
dc.contributor.alternativeNameHong, Soon Won-
dc.contributor.alternativeNameKim, Eung Kweon-
dc.contributor.alternativeNameLee, Jong Eun-
dc.contributor.affiliatedAuthorLee, Joon Haeng-
dc.contributor.affiliatedAuthorLee, Hyung Keun-
dc.contributor.affiliatedAuthorHong, Soon Won-
dc.contributor.affiliatedAuthorKim, Eung Kweon-
dc.contributor.affiliatedAuthorLee, Jong Eun-
dc.rights.accessRightsfree-
dc.citation.volume47-
dc.citation.number3-
dc.citation.startPage864-
dc.citation.endPage872-
dc.identifier.bibliographicCitationInvestigative Ophthalmology & Visual Science, Vol.47(3) : 864-872, 2006-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Ophthalmology (안과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Anatomy (해부학교실) > 1. Journal Papers

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